tetano
Editor, Senior Moderator
J Clin Virol. 2012 Aug 23. [Epub ahead of print]
Early detection of influenza A and B infection in infants and children using conventional and fluorescence-based rapid testing.
Rath B, Tief F, Obermeier P, Tuerk E, Karsch K, Muehlhans S, Adamou E, Duwe S, Schweiger B.
Source
Department of Paediatrics, Division of Pneumonology-Immunology, Charit? University Medical Centre, Augustenburger Platz 1, Berlin, Germany.
Abstract
BACKGROUND:
The appropriate management of infants and children with influenza depends on the accurate and timely diagnosis, ideally at the point of care (POC).
OBJECTIVES:
To evaluate the use of simultaneous RSV/influenza rapid testing with QuickVue? test strips as well as (the use of) novel, fluorescence-based, rapid influenza antigen testing (SOFIA?) in infants and children with influenza-like illness (ILI).
STUDY DESIGN:
The Study was conducted in a real-time surveillance program at the Charit? Department of Pediatrics in collaboration with the National Reference Centre for Influenza at the Robert Koch Institute (RKI) in Berlin, Germany (Charit? Influenza-Like Disease=ChILD Cohort).
RESULTS:
During the 2010/2011 influenza season, 395 infants and children were simultaneously tested using QuickVue? FluA&B and RSV10 rapid tests at POC compared to independent RT-PCR. Sensitivities were 62.7/67.8% for Influenza/RSV overall, but highest in infants <1 year with 76.0/76.2%. The evaluation of the fluorescence-based rapid test SOFIA? with frozen laboratory samples (derived from the 2008/2009 and 2010/2011 national surveillance) yielded sensitivities of 97.7/86.7/86.7/81.7% for influenza A(H1N1)pdm09/A(H3N2)/B-Victoria/B-Yamagata in samples with CT values <34, and 80.2/79.8/67.5/62.5% for all CT values combined. The same method used at POC with 649 consecutive ChILD patients in 2011-2012 yielded sensitivity/specificity/PPV/NPV values of 78.9/99.7/96.6/97.3%. Again, sensitivities were highest in infants (85.7%) and small children <2 years (88%).
CONCLUSIONS:
Fluorescence-based rapid antigen testing provides a highly sensitive and specific tool for POC diagnostics of acute influenza in the paediatric age group, especially in infants and small children <2 years, when viral loads are at their peak and treatment decisions are imminent.
Copyright ? 2012 Elsevier B.V. All rights reserved.
PMID:
22921515
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22921515
Early detection of influenza A and B infection in infants and children using conventional and fluorescence-based rapid testing.
Rath B, Tief F, Obermeier P, Tuerk E, Karsch K, Muehlhans S, Adamou E, Duwe S, Schweiger B.
Source
Department of Paediatrics, Division of Pneumonology-Immunology, Charit? University Medical Centre, Augustenburger Platz 1, Berlin, Germany.
Abstract
BACKGROUND:
The appropriate management of infants and children with influenza depends on the accurate and timely diagnosis, ideally at the point of care (POC).
OBJECTIVES:
To evaluate the use of simultaneous RSV/influenza rapid testing with QuickVue? test strips as well as (the use of) novel, fluorescence-based, rapid influenza antigen testing (SOFIA?) in infants and children with influenza-like illness (ILI).
STUDY DESIGN:
The Study was conducted in a real-time surveillance program at the Charit? Department of Pediatrics in collaboration with the National Reference Centre for Influenza at the Robert Koch Institute (RKI) in Berlin, Germany (Charit? Influenza-Like Disease=ChILD Cohort).
RESULTS:
During the 2010/2011 influenza season, 395 infants and children were simultaneously tested using QuickVue? FluA&B and RSV10 rapid tests at POC compared to independent RT-PCR. Sensitivities were 62.7/67.8% for Influenza/RSV overall, but highest in infants <1 year with 76.0/76.2%. The evaluation of the fluorescence-based rapid test SOFIA? with frozen laboratory samples (derived from the 2008/2009 and 2010/2011 national surveillance) yielded sensitivities of 97.7/86.7/86.7/81.7% for influenza A(H1N1)pdm09/A(H3N2)/B-Victoria/B-Yamagata in samples with CT values <34, and 80.2/79.8/67.5/62.5% for all CT values combined. The same method used at POC with 649 consecutive ChILD patients in 2011-2012 yielded sensitivity/specificity/PPV/NPV values of 78.9/99.7/96.6/97.3%. Again, sensitivities were highest in infants (85.7%) and small children <2 years (88%).
CONCLUSIONS:
Fluorescence-based rapid antigen testing provides a highly sensitive and specific tool for POC diagnostics of acute influenza in the paediatric age group, especially in infants and small children <2 years, when viral loads are at their peak and treatment decisions are imminent.
Copyright ? 2012 Elsevier B.V. All rights reserved.
PMID:
22921515
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22921515