tetano
Editor, Senior Moderator
Virology. 2014 Jul 28;464-465C:156-165. doi: 10.1016/j.virol.2014.07.004. [Epub ahead of print]
Effect of receptor binding specificity on the immunogenicity and protective efficacy of influenza virus A H1 vaccines.
Sun X1, Cao W1, Pappas C1, Liu F1, Katz JM1, Tumpey TM2.
Author information
Abstract
The biological basis for the poor immunogenicity of unadjuvanted avian influenza A virus vaccines in mammals is not well understood. Here, we mutated the hemagglutinin (HA) of two H1N1 virus vaccines to determine whether virus receptor binding specificity contributes to the low immunogenicity of avian influenza virus vaccines. Mutations were introduced into the HA of an avian influenza virus, A/Duck/New York/15024-21/96 (Dk/96) which switched the binding preference from α2,3- to α2,6-linked sialic acid (SA). A switch in receptor specificity of the human A/South Carolina/1/18 (SC/18) virus generated a mutant virus with α2,3 SA (avian) binding preference. Inactivated vaccines were generated and administered to mice and ferrets intramuscularly. We found that the vaccines with human receptor binding preference induced slightly higher antibody titers and cell-mediated immune responses compared to their isogenic viruses with avian receptor binding specificity. Upon challenge with DK/96 or SC18 virus, differences in lung virus titers between the vaccine groups with different receptor-binding specificities were minimal. Overall, our data suggest that receptor binding specificity contributes only marginally to the immunogenicity of avian influenza vaccines and that other factors may also be involved.
Published by Elsevier Inc.
KEYWORDS:
Immunogenicity; Inactivated whole virus vaccine; Influenza virus; Receptor binding specificity
PMID:
25078114
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25078114
Effect of receptor binding specificity on the immunogenicity and protective efficacy of influenza virus A H1 vaccines.
Sun X1, Cao W1, Pappas C1, Liu F1, Katz JM1, Tumpey TM2.
Author information
Abstract
The biological basis for the poor immunogenicity of unadjuvanted avian influenza A virus vaccines in mammals is not well understood. Here, we mutated the hemagglutinin (HA) of two H1N1 virus vaccines to determine whether virus receptor binding specificity contributes to the low immunogenicity of avian influenza virus vaccines. Mutations were introduced into the HA of an avian influenza virus, A/Duck/New York/15024-21/96 (Dk/96) which switched the binding preference from α2,3- to α2,6-linked sialic acid (SA). A switch in receptor specificity of the human A/South Carolina/1/18 (SC/18) virus generated a mutant virus with α2,3 SA (avian) binding preference. Inactivated vaccines were generated and administered to mice and ferrets intramuscularly. We found that the vaccines with human receptor binding preference induced slightly higher antibody titers and cell-mediated immune responses compared to their isogenic viruses with avian receptor binding specificity. Upon challenge with DK/96 or SC18 virus, differences in lung virus titers between the vaccine groups with different receptor-binding specificities were minimal. Overall, our data suggest that receptor binding specificity contributes only marginally to the immunogenicity of avian influenza vaccines and that other factors may also be involved.
Published by Elsevier Inc.
KEYWORDS:
Immunogenicity; Inactivated whole virus vaccine; Influenza virus; Receptor binding specificity
PMID:
25078114
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25078114