tetano
Editor, Senior Moderator
J Breath Res. 2011 Jul 15;5(3):037107. [Epub ahead of print]
Effect of the influenza A (H1N1) live attenuated intranasal vaccine on nitric oxide (FE(NO)) and other volatiles in exhaled breath.
Mashir A, Paschke KM, van Duin D, Shrestha NK, Laskowski D, Storer MK, Yen-Lieberman B, Gordon SM, Aytekin M, Dweik RA.
Source
Department of Pathobiology/Lerner Research Institute, Cleveland Clinic, 9500 Euclid Ave, Cleveland, OH 44195, USA.
Abstract
For the 2009 influenza A (H1N1) pandemic, vaccination and infection control were the main modes of prevention. A live attenuated H1N1 vaccine mimics natural infection and works by evoking a host immune response, but currently there are no easy methods to measure such a response. To determine if an immune response could be measured in exhaled breath, exhaled nitric oxide (FE(NO)) and other exhaled breath volatiles using selected ion flow tube mass spectrometry (SIFT-MS) were measured before and daily for seven days after administering the H1N1 2009 monovalent live intranasal vaccine (FluMist?, MedImmune LLC) in nine healthy healthcare workers (age 35 ? 7 years; five females). On day 3 after H1N1 FluMist? administration there were increases in FE(NO) (MEAN?SEM: day 0 15 ? 3 ppb, day 3 19 ? 3 ppb; p < 0.001) and breath isoprene (MEAN?SEM: day 0 59 ? 15 ppb, day 3 99 ? 17 ppb; p = 0.02). MS analysis identified the greatest number of changes in exhaled breath on day 3 with 137 product ion masses that changed from baseline. The exhaled breath changes on day 3 after H1N1 vaccination may reflect the underlying host immune response. However, further work to elucidate the sources of the exhaled breath changes is necessary.
PMID:
21757798
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21757798
Effect of the influenza A (H1N1) live attenuated intranasal vaccine on nitric oxide (FE(NO)) and other volatiles in exhaled breath.
Mashir A, Paschke KM, van Duin D, Shrestha NK, Laskowski D, Storer MK, Yen-Lieberman B, Gordon SM, Aytekin M, Dweik RA.
Source
Department of Pathobiology/Lerner Research Institute, Cleveland Clinic, 9500 Euclid Ave, Cleveland, OH 44195, USA.
Abstract
For the 2009 influenza A (H1N1) pandemic, vaccination and infection control were the main modes of prevention. A live attenuated H1N1 vaccine mimics natural infection and works by evoking a host immune response, but currently there are no easy methods to measure such a response. To determine if an immune response could be measured in exhaled breath, exhaled nitric oxide (FE(NO)) and other exhaled breath volatiles using selected ion flow tube mass spectrometry (SIFT-MS) were measured before and daily for seven days after administering the H1N1 2009 monovalent live intranasal vaccine (FluMist?, MedImmune LLC) in nine healthy healthcare workers (age 35 ? 7 years; five females). On day 3 after H1N1 FluMist? administration there were increases in FE(NO) (MEAN?SEM: day 0 15 ? 3 ppb, day 3 19 ? 3 ppb; p < 0.001) and breath isoprene (MEAN?SEM: day 0 59 ? 15 ppb, day 3 99 ? 17 ppb; p = 0.02). MS analysis identified the greatest number of changes in exhaled breath on day 3 with 137 product ion masses that changed from baseline. The exhaled breath changes on day 3 after H1N1 vaccination may reflect the underlying host immune response. However, further work to elucidate the sources of the exhaled breath changes is necessary.
PMID:
21757798
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21757798