tetano
Editor, Senior Moderator
Nan Fang Yi Ke Da Xue Xue Bao. 2012 Jul;32(7):966-9.
[Effect of total flavonoids of Scutellaria baicalensis Georgi on expression of influenza A virus nucleoprotein in HeLa cells].
[Article in Chinese]
Zhang Q, Yang B, Wang NR, Duan LJ, He SQ, Sun J.
Source
Fourth Affiliated Hospital of Nanchang University, Nanchang 330003, China.E-mail: qqzqzqz@yahoo.com.cn.
Abstract
OBJECTIVE:
To investigate the effect of total flavonoids of Scutellaria baicalensis Georgi (TFSB) on exogenous expression of influenza A virus nucleoprotein (NP) in HeLa cells.
METHODS:
HeLa cells were transiently transfected with the empty vector pcDNA3.1(+) or pcDNA3.1(+)/NP vector harboring influenza A virus NP. The pcDNA3.1(+)/NP-transfected cells were treated with TSFB and the expression of influenza A virus NP in the cell supernatant was measured using colloidal gold immunochromatography 48 h after the transfection; fluorescent quantitative RT-PCR was performed to measure the starting copy number of NP gene.
RESULTS:
The cells transfected with pcDNA3.1 (+)/NP with and without TFSB treatment were positive for NP expression. Fluorescent quantitative RT-PCR showed that the starting copy number of NP gene in pcDNA3.1(+)/NP-transfected cells was (8.90∓2.53)?10(6) copies/?l, showing no significant difference from that of (6.15∓1.49)?10(6) copies/?l in pcDNA3.1(+)/NP-transfected cells with subsequent TFSB treatment (P>0.05).
CONCLUSION:
TFSB treatment does not obviously affect exogenous influenza A virus NP gene expression or its protein synthesis in HeLa cells.
PMID:
22820579
[PubMed - in process]
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/22820579
[Effect of total flavonoids of Scutellaria baicalensis Georgi on expression of influenza A virus nucleoprotein in HeLa cells].
[Article in Chinese]
Zhang Q, Yang B, Wang NR, Duan LJ, He SQ, Sun J.
Source
Fourth Affiliated Hospital of Nanchang University, Nanchang 330003, China.E-mail: qqzqzqz@yahoo.com.cn.
Abstract
OBJECTIVE:
To investigate the effect of total flavonoids of Scutellaria baicalensis Georgi (TFSB) on exogenous expression of influenza A virus nucleoprotein (NP) in HeLa cells.
METHODS:
HeLa cells were transiently transfected with the empty vector pcDNA3.1(+) or pcDNA3.1(+)/NP vector harboring influenza A virus NP. The pcDNA3.1(+)/NP-transfected cells were treated with TSFB and the expression of influenza A virus NP in the cell supernatant was measured using colloidal gold immunochromatography 48 h after the transfection; fluorescent quantitative RT-PCR was performed to measure the starting copy number of NP gene.
RESULTS:
The cells transfected with pcDNA3.1 (+)/NP with and without TFSB treatment were positive for NP expression. Fluorescent quantitative RT-PCR showed that the starting copy number of NP gene in pcDNA3.1(+)/NP-transfected cells was (8.90∓2.53)?10(6) copies/?l, showing no significant difference from that of (6.15∓1.49)?10(6) copies/?l in pcDNA3.1(+)/NP-transfected cells with subsequent TFSB treatment (P>0.05).
CONCLUSION:
TFSB treatment does not obviously affect exogenous influenza A virus NP gene expression or its protein synthesis in HeLa cells.
PMID:
22820579
[PubMed - in process]
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/22820579