tetano
Editor, Senior Moderator
Environ Health Perspect. 2014 Jul 22. [Epub ahead of print]
Effects of Developmental Activation of the AhR on CD4+ T-Cell Responses to Influenza Virus Infection in Adult Mice.
Boule LA1, Winans B2, Lawrence BP3.
Author information
Abstract
BACKGROUND:
Epidemiological and animal studies indicate that maternal exposure to pollutants that bind the aryl hydrocarbon receptor (AhR) correlates with poorer ability to combat respiratory infection and lower antibody levels in the offspring. These observations point to an impact on CD4+ T cells. Yet, the consequence of developmental exposure to AhR ligands on the activation and differentiation of CD4+ T cells has not been directly examined.
OBJECTIVES:
Our goal was to determine whether maternal exposure to an AhR ligand directly alters CD4+ T cell differentiation and function later in life.
METHODS:
C57B1/6 mice were exposed to a prototypical AhR ligand, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in utero and via suckling. CD4+ T cell activation and differentiation into distinct effector populations was measured in adult offspring that were infected with influenza A virus (IAV). Reciprocal adoptive transfers were used to define whether modifications in CD4+ T cell responses resulted from direct effects of developmental exposure on CD4+ T cells.
RESULTS:
Developmental exposure skewed CD4+ T cell responses to IAV infection. There were fewer virus-specific, activated CD4+ T cells, and a reduced frequency of conventional CD4+ effector cell subsets. However, there was an increase in regulatory CD4+ T cells. Impaired differentiation into conventional effector subsets was due to direct effects of AhR activation on CD4+ T cells, as this defect can be transferred to mice that had not been developmentally exposed to TCDD.
CONCLUSIONS:
Maternal exposure to TCDD causes durable changes in the responsive capacity and differentiation of CD4+ T cells in adult C57Bl/6 mice.
PMID:
25051576
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25051576
Effects of Developmental Activation of the AhR on CD4+ T-Cell Responses to Influenza Virus Infection in Adult Mice.
Boule LA1, Winans B2, Lawrence BP3.
Author information
Abstract
BACKGROUND:
Epidemiological and animal studies indicate that maternal exposure to pollutants that bind the aryl hydrocarbon receptor (AhR) correlates with poorer ability to combat respiratory infection and lower antibody levels in the offspring. These observations point to an impact on CD4+ T cells. Yet, the consequence of developmental exposure to AhR ligands on the activation and differentiation of CD4+ T cells has not been directly examined.
OBJECTIVES:
Our goal was to determine whether maternal exposure to an AhR ligand directly alters CD4+ T cell differentiation and function later in life.
METHODS:
C57B1/6 mice were exposed to a prototypical AhR ligand, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in utero and via suckling. CD4+ T cell activation and differentiation into distinct effector populations was measured in adult offspring that were infected with influenza A virus (IAV). Reciprocal adoptive transfers were used to define whether modifications in CD4+ T cell responses resulted from direct effects of developmental exposure on CD4+ T cells.
RESULTS:
Developmental exposure skewed CD4+ T cell responses to IAV infection. There were fewer virus-specific, activated CD4+ T cells, and a reduced frequency of conventional CD4+ effector cell subsets. However, there was an increase in regulatory CD4+ T cells. Impaired differentiation into conventional effector subsets was due to direct effects of AhR activation on CD4+ T cells, as this defect can be transferred to mice that had not been developmentally exposed to TCDD.
CONCLUSIONS:
Maternal exposure to TCDD causes durable changes in the responsive capacity and differentiation of CD4+ T cells in adult C57Bl/6 mice.
PMID:
25051576
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25051576