tetano
Editor, Senior Moderator
Clin Vaccine Immunol. 2011 Dec 21. [Epub ahead of print]
Effects of different adjuvants in the context of intramuscular and intranasal routes on humoral and cellular immune responses induced by detergent-split A/H3N2 influenza vaccines in mice.
Baz M, Samant M, Zekki H, Tribout-Jover P, Plante M, Lanteigne AM, Hamelin ME, Mallett C, Papadopoulou B, Boivin G.
Source
Research Center in Infectious Diseases of the CHUL (CHUQ) and Department of Microbiology and Immunology of Laval University, Quebec, QC, Canada, G1V 4G2.
Abstract
Influenza A/H3N2 viruses have caused the most severe epidemics since 1968 despite current immunization programs with inactivated vaccines. We undertook a side-by-side pre-clinical evaluation of different adjuvants (Alum, AS03 and Protollin) and routes of administration (intramuscular (i.m.) and intranasal (i.n.)) for assessing their effect on the immunogenicity and cross-reactivity of inactivated split vaccines (A/H3N2/New York/55/2004). Humoral and T cell-mediated immune responses against the homologous virus and a heterologous drifted strain (A/H3N2/Wisconsin/67/2005) were measured in BALB/c mice at 2, 6, and 19 weeks post-boost. The AS03- and alum-adjuvanted i.m. vaccines induced at least an 8-fold increase over the unadjuvanted vaccine in functional antibody titers against both the homotypic and heterotypic strains and low IgG2a and high IgG1 levels, suggesting a mixed Th1/Th2 response with a Th2 trend. The Protollin-adjuvanted i.n. vaccine induced the lowest IgG1/IgG2a ratio, which is indicative of a mixed Th1/Th2-type profile with a Th1 trend. This adjuvanted vaccine was the only vaccine to stimulate a mucosal IgA response. Whatever the timing after the boost, both hemagglutination inhibition (HAI) and microneutralization (MN) titers were higher with the AS03-adjuvanted i.m. vaccine compared to the protollin-adjuvanted i.n. vaccine. Finally, the Alum-adjuvanted i.m. vaccine and the lower-dose Protollin-adjuvanted i.n. vaccine elicited significantly higher CD4+ Th1 and Th2 responses and more IFN-γ-producing CD8+ T cells than the non-adjuvanted vaccine. Our data indicate that the adjuvanted vaccines tested in this study can elicit stronger, more persistent and broader immune responses against A/H3N2 strains compared to unadjuvanted inactivated influenza vaccines.
PMID:
22190392
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22190392
Effects of different adjuvants in the context of intramuscular and intranasal routes on humoral and cellular immune responses induced by detergent-split A/H3N2 influenza vaccines in mice.
Baz M, Samant M, Zekki H, Tribout-Jover P, Plante M, Lanteigne AM, Hamelin ME, Mallett C, Papadopoulou B, Boivin G.
Source
Research Center in Infectious Diseases of the CHUL (CHUQ) and Department of Microbiology and Immunology of Laval University, Quebec, QC, Canada, G1V 4G2.
Abstract
Influenza A/H3N2 viruses have caused the most severe epidemics since 1968 despite current immunization programs with inactivated vaccines. We undertook a side-by-side pre-clinical evaluation of different adjuvants (Alum, AS03 and Protollin) and routes of administration (intramuscular (i.m.) and intranasal (i.n.)) for assessing their effect on the immunogenicity and cross-reactivity of inactivated split vaccines (A/H3N2/New York/55/2004). Humoral and T cell-mediated immune responses against the homologous virus and a heterologous drifted strain (A/H3N2/Wisconsin/67/2005) were measured in BALB/c mice at 2, 6, and 19 weeks post-boost. The AS03- and alum-adjuvanted i.m. vaccines induced at least an 8-fold increase over the unadjuvanted vaccine in functional antibody titers against both the homotypic and heterotypic strains and low IgG2a and high IgG1 levels, suggesting a mixed Th1/Th2 response with a Th2 trend. The Protollin-adjuvanted i.n. vaccine induced the lowest IgG1/IgG2a ratio, which is indicative of a mixed Th1/Th2-type profile with a Th1 trend. This adjuvanted vaccine was the only vaccine to stimulate a mucosal IgA response. Whatever the timing after the boost, both hemagglutination inhibition (HAI) and microneutralization (MN) titers were higher with the AS03-adjuvanted i.m. vaccine compared to the protollin-adjuvanted i.n. vaccine. Finally, the Alum-adjuvanted i.m. vaccine and the lower-dose Protollin-adjuvanted i.n. vaccine elicited significantly higher CD4+ Th1 and Th2 responses and more IFN-γ-producing CD8+ T cells than the non-adjuvanted vaccine. Our data indicate that the adjuvanted vaccines tested in this study can elicit stronger, more persistent and broader immune responses against A/H3N2 strains compared to unadjuvanted inactivated influenza vaccines.
PMID:
22190392
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22190392