tetano
Editor, Senior Moderator
Vaccine. 2012 Aug 22. [Epub ahead of print]
Effects of route and co-administration of recombinant raccoon poxviruses on immune responses and protection against highly pathogenic avian influenza in mice.
Kingstad-Bakke B, Brewoo JN, Mai LQ, Kawaoka Y, Osorio JE.
Source
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706, USA.
Abstract
We previously demonstrated that recombinant raccoonpox (RCN) virus could serve as a vector for an influenza vaccine. RCN constructs expressing the hemagglutinin (HA) from H5N1 viruses were immunogenic in chickens. In the current study, we generated several recombinant RCN constructs expressing influenza (H5N1) antigens and a molecular adjuvant (Heat-Labile enterotoxin B from E. coli: RCN-LTB), demonstrated their expression in vitro, and evaluated their ability to protect mice against H5N1 virus challenge. RCN-HA provided strong protection when administered intradermally (ID), but not intranasally (IN). Conversely, the RCN-neuraminidase (NA) construct was highly efficacious by the IN route and elicited high titers of neutralizing antibodies in mice. Vaccination by combined ID (RCN-HA) and IN (RCN-NA) routes offered mice the best protection against an IN challenge with heterologous H5N1 virus. However, protection was reduced when the different RCN constructs were pre-mixed, perhaps due to reduced expression of antigen.
Copyright ? 2012. Published by Elsevier Ltd.
PMID:
22921740
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22921740
Effects of route and co-administration of recombinant raccoon poxviruses on immune responses and protection against highly pathogenic avian influenza in mice.
Kingstad-Bakke B, Brewoo JN, Mai LQ, Kawaoka Y, Osorio JE.
Source
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706, USA.
Abstract
We previously demonstrated that recombinant raccoonpox (RCN) virus could serve as a vector for an influenza vaccine. RCN constructs expressing the hemagglutinin (HA) from H5N1 viruses were immunogenic in chickens. In the current study, we generated several recombinant RCN constructs expressing influenza (H5N1) antigens and a molecular adjuvant (Heat-Labile enterotoxin B from E. coli: RCN-LTB), demonstrated their expression in vitro, and evaluated their ability to protect mice against H5N1 virus challenge. RCN-HA provided strong protection when administered intradermally (ID), but not intranasally (IN). Conversely, the RCN-neuraminidase (NA) construct was highly efficacious by the IN route and elicited high titers of neutralizing antibodies in mice. Vaccination by combined ID (RCN-HA) and IN (RCN-NA) routes offered mice the best protection against an IN challenge with heterologous H5N1 virus. However, protection was reduced when the different RCN constructs were pre-mixed, perhaps due to reduced expression of antigen.
Copyright ? 2012. Published by Elsevier Ltd.
PMID:
22921740
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22921740