tetano
Editor, Senior Moderator
Emerg Infect Dis
. 2026 Nov;32(11):1879-1884.
doi: 10.3201/eid3211.261234.
Valentina Paracchini, Mauro Petrillo, Francisco Mayo Leon, Gerhard Buttinger, Sabrina Marciano, Marika Crimaudo, Calogero Terregino, Carolina Veneri, Giuseppina La Rosa, Elisabetta Suffredini, Clementina Cocuzza, Bernd M Gawlik, Tobias Wiesenthal, Ursula Vincent, Valentina Panzarin, Antonio Marchini
Highly pathogenic avian influenza A(H5Nx) viruses, particularly clade 2.3.4.4b, pose substantial global health risks. We combined and piloted 2 duplex reverse transcription digital PCRs to discriminate between avian and seasonal influenza viruses in wastewater. This approach enabled rapid, strain-specific detection to reduce ambiguity in environmental surveillance for influenza viruses.
Keywords: Avian influenza; HPAI A(H5N1) clade 2.3.4.4b; Italy; digital RT-PCR; influenza; influenza A virus; influenza virus monitoring; public health; respiratory infections; seasonal influenza; viruses; wastewater surveillance.
. 2026 Nov;32(11):1879-1884.
doi: 10.3201/eid3211.261234.
Differentiating Avian and Seasonal Influenza Viruses in Wastewater by Digital PCR
Valentina Paracchini, Mauro Petrillo, Francisco Mayo Leon, Gerhard Buttinger, Sabrina Marciano, Marika Crimaudo, Calogero Terregino, Carolina Veneri, Giuseppina La Rosa, Elisabetta Suffredini, Clementina Cocuzza, Bernd M Gawlik, Tobias Wiesenthal, Ursula Vincent, Valentina Panzarin, Antonio Marchini
- PMID: 42912387
- DOI: 10.3201/eid3211.261234
Abstract
Highly pathogenic avian influenza A(H5Nx) viruses, particularly clade 2.3.4.4b, pose substantial global health risks. We combined and piloted 2 duplex reverse transcription digital PCRs to discriminate between avian and seasonal influenza viruses in wastewater. This approach enabled rapid, strain-specific detection to reduce ambiguity in environmental surveillance for influenza viruses.
Keywords: Avian influenza; HPAI A(H5N1) clade 2.3.4.4b; Italy; digital RT-PCR; influenza; influenza A virus; influenza virus monitoring; public health; respiratory infections; seasonal influenza; viruses; wastewater surveillance.