tetano
Editor, Senior Moderator
Emerg Infect Dis
. 2022 Mar 29;28(5).
doi: 10.3201/eid2805.212488. Online ahead of print.
Estimating Relative Abundance of 2 SARS-CoV-2 Variants through Wastewater Surveillance at 2 Large Metropolitan Sites, United States
Alexander T Yu, Bridgette Hughes, Marlene K Wolfe, Tomas Leon, Dorothea Duong, Angela Rabe, Lauren C Kennedy, Sindhu Ravuri, Bradley J White, Krista R Wigginton, Alexandria B Boehm, Duc J Vugia
Abstract
Monitoring severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern (VOCs) is critical for public health management of coronavirus disease. Sequencing is resource-intensive and incompletely representative, and not all isolates can be sequenced. Because wastewater SARS-CoV-2 RNA concentrations correlate with coronavirus disease incidence in sewersheds, tracking VOCs through wastewater is appealing. We developed digital reverse transcription PCRs to monitor abundance of select mutations in Alpha and Delta VOCs in wastewater settled solids, applied these to July 2020-August 2021 samples from 2 large US metropolitan sewersheds, and compared results to estimates of VOC abundance from case isolate sequencing. Wastewater measurements tracked closely with case isolate estimates (Alpha, r[SUB]p[/SUB] 0.82-0.88; Delta, r[SUB]p[/SUB] 0.97). Mutations were detected in wastewater even at levels <5% of total SARS-CoV-2 RNA and in samples available 1-3 weeks before case isolate results. Wastewater variant monitoring should be strategically deployed to complement case isolate sequencing.
Keywords: COVID-19; SARS-CoV-2; United States; coronavirus disease; environmental monitoring; public health; respiratory infections; severe acute respiratory syndrome coronavirus 2; vaccine-preventable diseases; viruses; wastewater; zoonoses.
. 2022 Mar 29;28(5).
doi: 10.3201/eid2805.212488. Online ahead of print.
Estimating Relative Abundance of 2 SARS-CoV-2 Variants through Wastewater Surveillance at 2 Large Metropolitan Sites, United States
Alexander T Yu, Bridgette Hughes, Marlene K Wolfe, Tomas Leon, Dorothea Duong, Angela Rabe, Lauren C Kennedy, Sindhu Ravuri, Bradley J White, Krista R Wigginton, Alexandria B Boehm, Duc J Vugia
- PMID: 35349402
- DOI: 10.3201/eid2805.212488
Abstract
Monitoring severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern (VOCs) is critical for public health management of coronavirus disease. Sequencing is resource-intensive and incompletely representative, and not all isolates can be sequenced. Because wastewater SARS-CoV-2 RNA concentrations correlate with coronavirus disease incidence in sewersheds, tracking VOCs through wastewater is appealing. We developed digital reverse transcription PCRs to monitor abundance of select mutations in Alpha and Delta VOCs in wastewater settled solids, applied these to July 2020-August 2021 samples from 2 large US metropolitan sewersheds, and compared results to estimates of VOC abundance from case isolate sequencing. Wastewater measurements tracked closely with case isolate estimates (Alpha, r[SUB]p[/SUB] 0.82-0.88; Delta, r[SUB]p[/SUB] 0.97). Mutations were detected in wastewater even at levels <5% of total SARS-CoV-2 RNA and in samples available 1-3 weeks before case isolate results. Wastewater variant monitoring should be strategically deployed to complement case isolate sequencing.
Keywords: COVID-19; SARS-CoV-2; United States; coronavirus disease; environmental monitoring; public health; respiratory infections; severe acute respiratory syndrome coronavirus 2; vaccine-preventable diseases; viruses; wastewater; zoonoses.