tetano
Editor, Senior Moderator
Emerg Infect Dis
. 2020 Jun 19;26(9).
doi: 10.3201/eid2609.201495. Online ahead of print.
Isolation, Sequence, Infectivity, and Replication Kinetics of Severe Acute Respiratory Syndrome Coronavirus 2
Arinjay Banerjee, Jalees A Nasir, Patrick Budylowski, Lily Yip, Patryk Aftanas, Natasha Christie, Ayoob Ghalami, Kaushal Baid, Amogelang R Raphenya, Jeremy A Hirota, Matthew S Miller, Allison J McGeer, Mario Ostrowski, Robert A Kozak, Andrew G McArthur, Karen Mossman, Samira Mubareka
Abstract
Since its emergence in Wuhan, China, in December 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected ≈6 million persons worldwide. As SARS-CoV-2 spreads across the planet, we explored the range of human cells that can be infected by this virus. We isolated SARS-CoV-2 from 2 infected patients in Toronto, Canada; determined the genomic sequences; and identified single-nucleotide changes in representative populations of our virus stocks. We also tested a wide range of human immune cells for productive infection with SARS-CoV-2. We confirm that human primary peripheral blood mononuclear cells are not permissive for SARS-CoV-2. As SARS-CoV-2 continues to spread globally, it is essential to monitor single-nucleotide polymorphisms in the virus and to continue to isolate circulating viruses to determine viral genotype and phenotype by using in vitro and in vivo infection models.
Keywords: 2019 novel coronavirus disease; COVID-19; SARS-CoV-2; coronavirus disease; immune cells; isolation; phylogenetics; replication; respiratory infections; severe acute respiratory syndrome coronavirus 2; viruses; zoonoses.
. 2020 Jun 19;26(9).
doi: 10.3201/eid2609.201495. Online ahead of print.
Isolation, Sequence, Infectivity, and Replication Kinetics of Severe Acute Respiratory Syndrome Coronavirus 2
Arinjay Banerjee, Jalees A Nasir, Patrick Budylowski, Lily Yip, Patryk Aftanas, Natasha Christie, Ayoob Ghalami, Kaushal Baid, Amogelang R Raphenya, Jeremy A Hirota, Matthew S Miller, Allison J McGeer, Mario Ostrowski, Robert A Kozak, Andrew G McArthur, Karen Mossman, Samira Mubareka
- PMID: 32558639
- DOI: 10.3201/eid2609.201495
Abstract
Since its emergence in Wuhan, China, in December 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected ≈6 million persons worldwide. As SARS-CoV-2 spreads across the planet, we explored the range of human cells that can be infected by this virus. We isolated SARS-CoV-2 from 2 infected patients in Toronto, Canada; determined the genomic sequences; and identified single-nucleotide changes in representative populations of our virus stocks. We also tested a wide range of human immune cells for productive infection with SARS-CoV-2. We confirm that human primary peripheral blood mononuclear cells are not permissive for SARS-CoV-2. As SARS-CoV-2 continues to spread globally, it is essential to monitor single-nucleotide polymorphisms in the virus and to continue to isolate circulating viruses to determine viral genotype and phenotype by using in vitro and in vivo infection models.
Keywords: 2019 novel coronavirus disease; COVID-19; SARS-CoV-2; coronavirus disease; immune cells; isolation; phylogenetics; replication; respiratory infections; severe acute respiratory syndrome coronavirus 2; viruses; zoonoses.