tetano
Editor, Senior Moderator
Emerg Infect Dis
. 2020 Jul 1;26(10).
doi: 10.3201/eid2610.201800. Online ahead of print.
Rapid, Sensitive, Full-Genome Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2
Clinton R Paden, Ying Tao, Krista Queen, Jing Zhang, Yan Li, Anna Uehara, Suxiang Tong
Abstract
We describe validated protocols for generating high-quality, full-length severe acute respiratory syndrome coronavirus 2 genomes from primary samples. One protocol uses multiplex reverse transcription PCR, followed by MinION or MiSeq sequencing; the other uses singleplex, nested reverse transcription PCR and Sanger sequencing. These protocols enable sensitive virus sequencing in different laboratory environments.
Keywords: COVID-19; SARS-CoV-2; coronavirus; coronavirus disease; genomics; high-throughput nucleotide sequencing; respiratory infections; severe acute respiratory syndrome coronavirus 2; viruses; whole-genome sequencing; zoonoses.
. 2020 Jul 1;26(10).
doi: 10.3201/eid2610.201800. Online ahead of print.
Rapid, Sensitive, Full-Genome Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2
Clinton R Paden, Ying Tao, Krista Queen, Jing Zhang, Yan Li, Anna Uehara, Suxiang Tong
- PMID: 32610037
- DOI: 10.3201/eid2610.201800
Abstract
We describe validated protocols for generating high-quality, full-length severe acute respiratory syndrome coronavirus 2 genomes from primary samples. One protocol uses multiplex reverse transcription PCR, followed by MinION or MiSeq sequencing; the other uses singleplex, nested reverse transcription PCR and Sanger sequencing. These protocols enable sensitive virus sequencing in different laboratory environments.
Keywords: COVID-19; SARS-CoV-2; coronavirus; coronavirus disease; genomics; high-throughput nucleotide sequencing; respiratory infections; severe acute respiratory syndrome coronavirus 2; viruses; whole-genome sequencing; zoonoses.