tetano
Editor, Senior Moderator
Emerg Infect Dis
. 2021 Feb;27(2):632-635.
doi: 10.3201/eid2702.204168.
Strand-Specific Reverse Transcription PCR for Detection of Replicating SARS-CoV-2
Catherine A Hogan, ChunHong Huang, Malaya K Sahoo, Hannah Wang, Becky Jiang, Mamdouh Sibai, Marisa Holubar, Roshni Mathew, James Zehnder, Benjamin A Pinsky
Abstract
We developed an assay that detects minus-strand RNA as a surrogate for actively replicating severe acute respiratory syndrome coronavirus 2. We detected minus-strand RNA in 41 persons with coronavirus disease up to 30 days after symptom onset. This assay might inform clinical decision-making about patient infectiousness.
Keywords: 2019 novel coronavirus disease; COVID-19; PCR; RNA; SARS-CoV-2; coronavirus; coronavirus disease; diagnosis; infection control; minus strand; respiratory infections; screening; severe acute respiratory syndrome coronavirus 2; testing; viruses; zoonoses.
. 2021 Feb;27(2):632-635.
doi: 10.3201/eid2702.204168.
Strand-Specific Reverse Transcription PCR for Detection of Replicating SARS-CoV-2
Catherine A Hogan, ChunHong Huang, Malaya K Sahoo, Hannah Wang, Becky Jiang, Mamdouh Sibai, Marisa Holubar, Roshni Mathew, James Zehnder, Benjamin A Pinsky
- PMID: 33496233
- DOI: 10.3201/eid2702.204168
Abstract
We developed an assay that detects minus-strand RNA as a surrogate for actively replicating severe acute respiratory syndrome coronavirus 2. We detected minus-strand RNA in 41 persons with coronavirus disease up to 30 days after symptom onset. This assay might inform clinical decision-making about patient infectiousness.
Keywords: 2019 novel coronavirus disease; COVID-19; PCR; RNA; SARS-CoV-2; coronavirus; coronavirus disease; diagnosis; infection control; minus strand; respiratory infections; screening; severe acute respiratory syndrome coronavirus 2; testing; viruses; zoonoses.