tetano
Editor, Senior Moderator
Emerg Microbes Infect
. 2021 Jan 7;1-24.
doi: 10.1080/22221751.2021.1873073. Online ahead of print.
Novel Dual Multiplex Real-time RT-PCR Assays for the Rapid Detection of SARS-CoV-2, Influenza A/B, and Respiratory Syncytial Virus using the BD Max Open System
Hsing-Yi Chung[SUP] 1 [/SUP], Ming-Jr Jian[SUP] 1 [/SUP], Chih-Kai Chang[SUP] 1 [/SUP], Jung-Chung Lin[SUP] 2 [/SUP], Kuo-Ming Yeh[SUP] 2 [/SUP], Chien-Wen Chen[SUP] 3 [/SUP], Sheng-Kang Chiu[SUP] 2 [/SUP], Yi-Hui Wang[SUP] 1 [/SUP], Shu-Jung Liao[SUP] 1 [/SUP], Shih-Yi Li[SUP] 1 [/SUP], Shan-Shan Hsieh[SUP] 1 [/SUP], Shih-Hung Tsai[SUP] 4 [/SUP], Cherng-Lih Perng[SUP] 1 [/SUP], Ji-Rong Yang[SUP] 5 [/SUP], Ming-Tsan Liu[SUP] 5 [/SUP], Feng-Yee Chang[SUP] 2 [/SUP], Hung-Sheng Shang[SUP] 1 [/SUP]
Affiliations
Abstract
Severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) has spread rapidly, causing deaths worldwide. In this study, we evaluated the performance of the BD MAX Open System module for identifying viral pathogens, including SARS-CoV-2, in nasopharyngeal specimens from individuals with symptoms of upper respiratory tract infection. We developed and validated a rapid total nucleic acid extraction method based on real-time reverse transcription-polymerase chain reaction (RT-PCR) for the reliable, high-throughput simultaneous detection of common cold viral pathogens using the BD MAX Platform. The system was evaluated using 205 nasopharyngeal swab clinical samples. For assessment of the limit of detection (LoD), we used SARS-CoV-2, influenza A/B, and respiratory syncytial virus (RSV) RNA standards. The BD MAX dual multiplex real-time RT-PCR panel demonstrated a sensitivity comparable to that of the World Health Organization-recommended SARS-CoV-2 assay with an LoD of 50 copies/PCR. The LoD of influenza A/B and RSV was 100-200 copies/PCR. The overall percent agreement between the BD MAX panel and laboratory-developed RT-PCR test on 55 SARS-CoV-2-positive clinical samples was 100%; no cross-reactions were encountered. Among the 55 positive cases of COVID-19 analysed, no coinfection was detected. The BD MAX rapid multiplex PCR provides a highly sensitive, robust, and accurate assay for the rapid detection of SARS-CoV-2, influenza A/B, and RSV. Our assay could accurately identify SARS-CoV-2 and other common respiratory viral infections, shortening the turnaround time, and could thus increase the effectiveness of control and prevention measures for this emerging infectious disease.
Keywords: BD MAX Platform; COVID-19; SARS-CoV-2; Taiwan; influenza; real-time PCR; respiratory syncytial virus; simultaneous detection.
. 2021 Jan 7;1-24.
doi: 10.1080/22221751.2021.1873073. Online ahead of print.
Novel Dual Multiplex Real-time RT-PCR Assays for the Rapid Detection of SARS-CoV-2, Influenza A/B, and Respiratory Syncytial Virus using the BD Max Open System
Hsing-Yi Chung[SUP] 1 [/SUP], Ming-Jr Jian[SUP] 1 [/SUP], Chih-Kai Chang[SUP] 1 [/SUP], Jung-Chung Lin[SUP] 2 [/SUP], Kuo-Ming Yeh[SUP] 2 [/SUP], Chien-Wen Chen[SUP] 3 [/SUP], Sheng-Kang Chiu[SUP] 2 [/SUP], Yi-Hui Wang[SUP] 1 [/SUP], Shu-Jung Liao[SUP] 1 [/SUP], Shih-Yi Li[SUP] 1 [/SUP], Shan-Shan Hsieh[SUP] 1 [/SUP], Shih-Hung Tsai[SUP] 4 [/SUP], Cherng-Lih Perng[SUP] 1 [/SUP], Ji-Rong Yang[SUP] 5 [/SUP], Ming-Tsan Liu[SUP] 5 [/SUP], Feng-Yee Chang[SUP] 2 [/SUP], Hung-Sheng Shang[SUP] 1 [/SUP]
Affiliations
- PMID: 33410371
- DOI: 10.1080/22221751.2021.1873073
Abstract
Severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) has spread rapidly, causing deaths worldwide. In this study, we evaluated the performance of the BD MAX Open System module for identifying viral pathogens, including SARS-CoV-2, in nasopharyngeal specimens from individuals with symptoms of upper respiratory tract infection. We developed and validated a rapid total nucleic acid extraction method based on real-time reverse transcription-polymerase chain reaction (RT-PCR) for the reliable, high-throughput simultaneous detection of common cold viral pathogens using the BD MAX Platform. The system was evaluated using 205 nasopharyngeal swab clinical samples. For assessment of the limit of detection (LoD), we used SARS-CoV-2, influenza A/B, and respiratory syncytial virus (RSV) RNA standards. The BD MAX dual multiplex real-time RT-PCR panel demonstrated a sensitivity comparable to that of the World Health Organization-recommended SARS-CoV-2 assay with an LoD of 50 copies/PCR. The LoD of influenza A/B and RSV was 100-200 copies/PCR. The overall percent agreement between the BD MAX panel and laboratory-developed RT-PCR test on 55 SARS-CoV-2-positive clinical samples was 100%; no cross-reactions were encountered. Among the 55 positive cases of COVID-19 analysed, no coinfection was detected. The BD MAX rapid multiplex PCR provides a highly sensitive, robust, and accurate assay for the rapid detection of SARS-CoV-2, influenza A/B, and RSV. Our assay could accurately identify SARS-CoV-2 and other common respiratory viral infections, shortening the turnaround time, and could thus increase the effectiveness of control and prevention measures for this emerging infectious disease.
Keywords: BD MAX Platform; COVID-19; SARS-CoV-2; Taiwan; influenza; real-time PCR; respiratory syncytial virus; simultaneous detection.