Endogenous Viral Elements in Animal Genomes

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PlosGenetics

Endogenous Viral Elements in Animal Genomes


Aris Katzourakis1*, Robert J. Gifford2*

1 Department of Zoology, University of Oxford, Oxford, United Kingdom, 2 Aaron Diamond AIDS Research Center, New York, New York, United States of America
Abstract Top

Integration into the nuclear genome of germ line cells can lead to vertical inheritance of retroviral genes as host alleles. For other viruses, germ line integration has only rarely been documented. Nonetheless, we identified endogenous viral elements (EVEs) derived from ten non-retroviral families by systematic in silico screening of animal genomes, including the first endogenous representatives of double-stranded RNA, reverse-transcribing DNA, and segmented RNA viruses, and the first endogenous DNA viruses in mammalian genomes. Phylogenetic and genomic analysis of EVEs across multiple host species revealed novel information about the origin and evolution of diverse virus groups. Furthermore, several of the elements identified here encode intact open reading frames or are expressed as mRNA. For one element in the primate lineage, we provide statistically robust evidence for exaptation. Our findings establish that genetic material derived from all known viral genome types and replication strategies can enter the animal germ line, greatly broadening the scope of paleovirological studies and indicating a more significant evolutionary role for gene flow from virus to animal genomes than has previously been recognized.

http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1001191

 

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Re: Endogenous Viral Elements in Animal Genomes

Paleovirology expanded: Non-retroviral virus fragments found in animal genomes

Understanding the evolution of life-threatening viruses like influenza, Ebola and dengue fever, could help us to minimize their impact. New research points the way to a fossil record of viruses that have insinuated themselves into the genomes of insects and other animals, providing clues about their evolutionary history. The findings, published online on November 18 in the open-access journal PLoS Genetics, could enable scientists to elucidate general principles in virus evolution from their genetic fossils, which in turn could inform approaches for controlling disease.

Robert Gifford (Rockefeller University), and colleague Aris Katzourakis (Oxford University) used the rapidly advancing technology for genetic screening to analyze a database of insect, bird and mammal DNA for fragments of virus genomes, which they named endogenous viral elements (EVEs). The pair discovered representatives of ten families of viruses, including hepatitis B, Ebola, rabies, and dengue and yellow fevers integrated into the genomes studied.

In some cases, weve got the first evidence of an ancient origin for some of these virus groups, said Gifford. While scientists have been aware of genetic signatures for retroviruses in animal genomes since the 1970s, much remains to be learned about these newly-discovered EVEs from non-retroviral viruses, including exactly how these virus fragments find their way into nuclear DNA.

Most of the fragments documented by Gifford are no longer functional, appearing like what is commonly referred to as junk DNA. However, the findings suggest that some of these virus fragments may have been co-opted by their hosts at some point in their evolutionary history, perhaps as a defense against related infections. In particular, Gifford says that finding EVEs in insect genomes promises to reveal a new dimension in paleovirology, allowing scientists to probe the relationship and evolution of the virus, its vector and its host, potentially providing insight into the complex ecological relationships that underpin insect-borne diseases.

http://www.webnewswire.com/node/625589