tetano
Editor, Senior Moderator
Biomed Microdevices. 2015 Jun;17(3):9951. doi: 10.1007/s10544-015-9951-z.
[h=1]Engineered self-assembling monolayers for label free detection of influenza nucleoprotein.[/h] Le Brun AP[SUP]1[/SUP], Soliakov A, Shah DS, Holt SA, McGill A, Lakey JH.
[h=3]Author information[/h]
[h=3]Abstract[/h] Integrating nanotechnology into useable devices requires a combination of bottom up and top down methodology. Often the techniques to measure and control these different components are entirely different, so methods that can analyse the nanoscale component in situ are of increasing importance. Here we describe a strategy that employs a self-assembling monolayer of engineered protein chimeras to display an array of oriented antibodies (IgG) on a microelectronic device for the label free detection of influenza nucleoprotein. The structural and functional properties of the bio-interface were characterised by a range of physical techniques including surface plasmon resonance, quartz-crystal microbalance and neutron reflectometry. This combination of methods reveals a 13.5 nm thick engineered-monolayer that (i) self-assembles on gold surfaces, (ii) captures IgG with high affinity in a defined orientation and (iii) specifically recognises the influenza A nucleoprotein. Furthermore we also show that this non-covalent self-assembled structure can render the dissociation of bound IgG irreversible by chemical crosslinking in situ without affecting the IgG function. The methods can thus describe in detail the transition from soluble engineered molecules with nanometre dimensions to an array that demonstrates the principles of a working influenza sensor.
PMID: 25860669 [PubMed - in process] PMCID: PMC4392172 Free PMC Article
[h=1]Engineered self-assembling monolayers for label free detection of influenza nucleoprotein.[/h] Le Brun AP[SUP]1[/SUP], Soliakov A, Shah DS, Holt SA, McGill A, Lakey JH.
[h=3]Author information[/h]
[h=3]Abstract[/h] Integrating nanotechnology into useable devices requires a combination of bottom up and top down methodology. Often the techniques to measure and control these different components are entirely different, so methods that can analyse the nanoscale component in situ are of increasing importance. Here we describe a strategy that employs a self-assembling monolayer of engineered protein chimeras to display an array of oriented antibodies (IgG) on a microelectronic device for the label free detection of influenza nucleoprotein. The structural and functional properties of the bio-interface were characterised by a range of physical techniques including surface plasmon resonance, quartz-crystal microbalance and neutron reflectometry. This combination of methods reveals a 13.5 nm thick engineered-monolayer that (i) self-assembles on gold surfaces, (ii) captures IgG with high affinity in a defined orientation and (iii) specifically recognises the influenza A nucleoprotein. Furthermore we also show that this non-covalent self-assembled structure can render the dissociation of bound IgG irreversible by chemical crosslinking in situ without affecting the IgG function. The methods can thus describe in detail the transition from soluble engineered molecules with nanometre dimensions to an array that demonstrates the principles of a working influenza sensor.
PMID: 25860669 [PubMed - in process] PMCID: PMC4392172 Free PMC Article