tetano
Editor, Senior Moderator
Viral Immunol. 2017 Nov 13. doi: 10.1089/vim.2017.0086. [Epub ahead of print]
[h=1]Enhanced CD103 Expression and Reduced Frequencies of Virus-Specific CD8+ T Cells Among Airway Lymphocytes After Influenza Vaccination of Mice Deficient in Vitamins A + D.[/h] Surman SL[SUP]1[/SUP], Jones BG[SUP]1[/SUP], Woodland DL[SUP]2[/SUP], Hurwitz JL[SUP]1,[/SUP][SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Previous research has evaluated antibody responses toward an influenza virus vaccine in the context of deficiencies for vitamins A and D (VAD+VDD). Results showed that antibodies and antibody-forming cells in the respiratory tract were reduced in VAD+VDD mice. However, effectors were recovered when oral supplements of vitamins A + D were delivered at the time of vaccination. Here we address the question of how vaccine-induced CD8[SUP]+[/SUP] T cell responses are affected by deficiencies for vitamins A + D. VAD+VDD and control mice were vaccinated with an intranasal, cold-adapted influenza virus A/Puerto Rico/8/34 vaccine, with or without oral supplements of vitamins A + D. Results showed that the percentages of vaccine-induced CD8[SUP]+[/SUP] T cell and total CD4[SUP]+[/SUP] T cell responses were low among lymphocytes in the airways of VAD+VDD animals compared to controls. The CD103 membrane marker, a protein that binds e-cadherin (expressed on respiratory tract epithelial cells), was unusually high on virus-specific T cells in VAD+VDD mice compared to controls. Interestingly, when T cells specific for the PA[SUB]224-233[/SUB]/D[SUP]b[/SUP] epitope were compared with T cells specific for the NP[SUB]366-374[/SUB]/D[SUP]b[/SUP] epitope, the former population was more strongly positive for CD103. Preliminary experiments revealed normal or above-normal percentages for vaccine-induced T cells in airways when VAD+VDD animals were supplemented with vitamins A + D at the time of vaccination and on days 3 and 7 after vaccination. Our results suggest that close attention should be paid to levels of vitamins A and D among vaccine recipients in the clinical arena, as low vitamin levels may render individuals poorly responsive to vaccines.
[h=4]KEYWORDS:[/h] bronchoalveolar lavage; influenza virus; vaccine-induced T cells; vitamin deficiency; vitamins A and D
PMID: 29130830 DOI: 10.1089/vim.2017.0086
[h=1]Enhanced CD103 Expression and Reduced Frequencies of Virus-Specific CD8+ T Cells Among Airway Lymphocytes After Influenza Vaccination of Mice Deficient in Vitamins A + D.[/h] Surman SL[SUP]1[/SUP], Jones BG[SUP]1[/SUP], Woodland DL[SUP]2[/SUP], Hurwitz JL[SUP]1,[/SUP][SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Previous research has evaluated antibody responses toward an influenza virus vaccine in the context of deficiencies for vitamins A and D (VAD+VDD). Results showed that antibodies and antibody-forming cells in the respiratory tract were reduced in VAD+VDD mice. However, effectors were recovered when oral supplements of vitamins A + D were delivered at the time of vaccination. Here we address the question of how vaccine-induced CD8[SUP]+[/SUP] T cell responses are affected by deficiencies for vitamins A + D. VAD+VDD and control mice were vaccinated with an intranasal, cold-adapted influenza virus A/Puerto Rico/8/34 vaccine, with or without oral supplements of vitamins A + D. Results showed that the percentages of vaccine-induced CD8[SUP]+[/SUP] T cell and total CD4[SUP]+[/SUP] T cell responses were low among lymphocytes in the airways of VAD+VDD animals compared to controls. The CD103 membrane marker, a protein that binds e-cadherin (expressed on respiratory tract epithelial cells), was unusually high on virus-specific T cells in VAD+VDD mice compared to controls. Interestingly, when T cells specific for the PA[SUB]224-233[/SUB]/D[SUP]b[/SUP] epitope were compared with T cells specific for the NP[SUB]366-374[/SUB]/D[SUP]b[/SUP] epitope, the former population was more strongly positive for CD103. Preliminary experiments revealed normal or above-normal percentages for vaccine-induced T cells in airways when VAD+VDD animals were supplemented with vitamins A + D at the time of vaccination and on days 3 and 7 after vaccination. Our results suggest that close attention should be paid to levels of vitamins A and D among vaccine recipients in the clinical arena, as low vitamin levels may render individuals poorly responsive to vaccines.
[h=4]KEYWORDS:[/h] bronchoalveolar lavage; influenza virus; vaccine-induced T cells; vitamin deficiency; vitamins A and D
PMID: 29130830 DOI: 10.1089/vim.2017.0086