tetano
Editor, Senior Moderator
Environ Microbiol
. 2022 Aug 3.
doi: 10.1111/1462-2920.16151. Online ahead of print.
Saliva for molecular detection of SARS-CoV-2 in pre-school and school-age children
Agnes Delaunay-Moisan[SUP] 1 [/SUP], Tiffany Guilleminot[SUP] 2 [/SUP], Michaela Semeraro[SUP] 3 [/SUP], Nelly Briand[SUP] 3 [/SUP], Brigitte Bader-Meunier[SUP] 4 5 [/SUP], Romain Berthaud[SUP] 3 [/SUP], Guillaume Morelle[SUP] 6 [/SUP], Pierre Quartier[SUP] 4 [/SUP], Caroline Galeotti[SUP] 6 [/SUP], Romain Basmaci[SUP] 7 [/SUP], Gregoire Benoist[SUP] 8 [/SUP], Vincent Gajdos[SUP] 9 [/SUP], Mathie Lorrot[SUP] 10 [/SUP], Mahmoud Rifai[SUP] 11 [/SUP], Matis Crespin[SUP] 11 [/SUP], Zakary M'Sakni[SUP] 11 [/SUP], Faheemah Padavia[SUP] 3 [/SUP], Catherine Savetier-Leroy[SUP] 12 [/SUP], Michelle Lorenzi[SUP] 12 [/SUP], Caroline Maurin[SUP] 12 [/SUP], Sylvie Behillil[SUP] 13 [/SUP], Loic de Pontual[SUP] 14 [/SUP], Narcisse Elenga[SUP] 15 [/SUP], Naim Bouazza[SUP] 3 [/SUP], Brigitte Moltrecht[SUP] 12 [/SUP], Sylvie van der Werf[SUP] 13 [/SUP], Marianne Leruez-Ville[SUP] 2 [/SUP], Isabelle Sermet-Gaudelus[SUP] 11 16 17 [/SUP]
Affiliations
Abstract
SARS-CoV-2 diagnosis is a cornerstone for the management of coronavirus disease 2019 (COVID-19). Numerous studies have assessed saliva performance over nasopharyngeal sampling (NPS), but data in young children are still rare. We explored saliva performance for SARS-CoV-2 detection by RT-PCR according to the time interval from initial symptoms or patient serological status. We collected 509 NPS and saliva paired samples at initial diagnosis from 166 children under 12 years of age (including 57 children under 6), 106 between 12 and 17, and 237 adults. In children under 12, overall detection rate for SARS-CoV-2 was comparable in saliva and NPS, with an overall agreement of 89.8%. Saliva sensitivity was significantly lower than that of NPS (77.1% compared to 95.8%) in pre-school and school-age children but regained 96% when considering seronegative children only. This pattern was also observed to a lesser degree in adolescents but not in adults. Sensitivity of saliva was independent of symptoms, in contrary to NPS, whose sensitivity decreased significantly in asymptomatic subjects. Performance of saliva is excellent in children under 12 at early stages of infection. This reinforces saliva as a collection method for early and unbiased SARS-CoV-2 detection and a less invasive alternative for young children.
. 2022 Aug 3.
doi: 10.1111/1462-2920.16151. Online ahead of print.
Saliva for molecular detection of SARS-CoV-2 in pre-school and school-age children
Agnes Delaunay-Moisan[SUP] 1 [/SUP], Tiffany Guilleminot[SUP] 2 [/SUP], Michaela Semeraro[SUP] 3 [/SUP], Nelly Briand[SUP] 3 [/SUP], Brigitte Bader-Meunier[SUP] 4 5 [/SUP], Romain Berthaud[SUP] 3 [/SUP], Guillaume Morelle[SUP] 6 [/SUP], Pierre Quartier[SUP] 4 [/SUP], Caroline Galeotti[SUP] 6 [/SUP], Romain Basmaci[SUP] 7 [/SUP], Gregoire Benoist[SUP] 8 [/SUP], Vincent Gajdos[SUP] 9 [/SUP], Mathie Lorrot[SUP] 10 [/SUP], Mahmoud Rifai[SUP] 11 [/SUP], Matis Crespin[SUP] 11 [/SUP], Zakary M'Sakni[SUP] 11 [/SUP], Faheemah Padavia[SUP] 3 [/SUP], Catherine Savetier-Leroy[SUP] 12 [/SUP], Michelle Lorenzi[SUP] 12 [/SUP], Caroline Maurin[SUP] 12 [/SUP], Sylvie Behillil[SUP] 13 [/SUP], Loic de Pontual[SUP] 14 [/SUP], Narcisse Elenga[SUP] 15 [/SUP], Naim Bouazza[SUP] 3 [/SUP], Brigitte Moltrecht[SUP] 12 [/SUP], Sylvie van der Werf[SUP] 13 [/SUP], Marianne Leruez-Ville[SUP] 2 [/SUP], Isabelle Sermet-Gaudelus[SUP] 11 16 17 [/SUP]
Affiliations
- PMID: 36065993
- DOI: 10.1111/1462-2920.16151
Abstract
SARS-CoV-2 diagnosis is a cornerstone for the management of coronavirus disease 2019 (COVID-19). Numerous studies have assessed saliva performance over nasopharyngeal sampling (NPS), but data in young children are still rare. We explored saliva performance for SARS-CoV-2 detection by RT-PCR according to the time interval from initial symptoms or patient serological status. We collected 509 NPS and saliva paired samples at initial diagnosis from 166 children under 12 years of age (including 57 children under 6), 106 between 12 and 17, and 237 adults. In children under 12, overall detection rate for SARS-CoV-2 was comparable in saliva and NPS, with an overall agreement of 89.8%. Saliva sensitivity was significantly lower than that of NPS (77.1% compared to 95.8%) in pre-school and school-age children but regained 96% when considering seronegative children only. This pattern was also observed to a lesser degree in adolescents but not in adults. Sensitivity of saliva was independent of symptoms, in contrary to NPS, whose sensitivity decreased significantly in asymptomatic subjects. Performance of saliva is excellent in children under 12 at early stages of infection. This reinforces saliva as a collection method for early and unbiased SARS-CoV-2 detection and a less invasive alternative for young children.