tetano
Editor, Senior Moderator
Epidemiol Infect
. 2021 Jun 24;149:e153.
doi: 10.1017/S0950268821001369.
SARS-CoV-2 outbreak in a synagogue community: longevity and strength of anti-SARS-CoV-2 IgG responses
Yael Gozlan[SUP] 1 [/SUP], Stephen Reingold[SUP] 2 [/SUP], Ravit Koren[SUP] 1 [/SUP], Osnat Halpern[SUP] 1 [/SUP], Gili Regev-Yochay[SUP] 3 [/SUP], Carmit Cohen[SUP] 3 [/SUP], Asaf Biber[SUP] 3 [/SUP], Orit Picard[SUP] 4 [/SUP], Ella Mendelson[SUP] 1 5 [/SUP], Yaniv Lustig[SUP] 1 5 [/SUP], Orna Mor[SUP] 1 5 [/SUP]
Affiliations
Abstract
Severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) pandemic is still ongoing along with the global vaccination efforts against it. Here, we aimed to understand the longevity and strength of anti-SARS-CoV-2 IgG responses in a small community (n = 283) six months following local SARS-COV-2 outbreak in March 2020. Three serological assays were compared and neutralisation capability was also determined. Overall 16.6% (47/283) of the participants were seropositive and 89.4% (42/47) of the IgG positives had neutralising antibodies. Most of the symptomatic individuals confirmed as polymerase chain reaction (PCR) positive during the outbreak were seropositive (30/32, 93.8%) and 33.3% of the individuals who quarantined with a PCR confirmed patient had antibodies. Serological assays comparison revealed that Architect (Abbott) targeting the N protein LIASON® (DiaSorin) targeting the S protein and enzyme-linked immunosorbent assay (ELISA) targeting receptor binding domain detected 9.5% (27/283), 17.3% (49/283) and 17% (48/283), respectively, as IgG positives. The latter two assays highly agreed (kappa = 0.89) between each other. In addition, 95%, (19/20, by ELISA) and 90.9% (20/22, with LIASON) and only 71.4% (15/21, by Architect) of individuals that were seropositive in May 2020 were found positive also in September. The unexpected low rate of overall immunity indicates the absence of un-noticed, asymptomatic infections. Lack of overall high correlation between the assays is attributed mainly to target-mediated antibody responses and suggests that using a single serological assay may be misleading.
Keywords: Herd immunity; IgG antibodies; SARS-CoV-2; neutralising antibodies; sero-prevalence.
. 2021 Jun 24;149:e153.
doi: 10.1017/S0950268821001369.
SARS-CoV-2 outbreak in a synagogue community: longevity and strength of anti-SARS-CoV-2 IgG responses
Yael Gozlan[SUP] 1 [/SUP], Stephen Reingold[SUP] 2 [/SUP], Ravit Koren[SUP] 1 [/SUP], Osnat Halpern[SUP] 1 [/SUP], Gili Regev-Yochay[SUP] 3 [/SUP], Carmit Cohen[SUP] 3 [/SUP], Asaf Biber[SUP] 3 [/SUP], Orit Picard[SUP] 4 [/SUP], Ella Mendelson[SUP] 1 5 [/SUP], Yaniv Lustig[SUP] 1 5 [/SUP], Orna Mor[SUP] 1 5 [/SUP]
Affiliations
- PMID: 34372950
- DOI: 10.1017/S0950268821001369
Abstract
Severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) pandemic is still ongoing along with the global vaccination efforts against it. Here, we aimed to understand the longevity and strength of anti-SARS-CoV-2 IgG responses in a small community (n = 283) six months following local SARS-COV-2 outbreak in March 2020. Three serological assays were compared and neutralisation capability was also determined. Overall 16.6% (47/283) of the participants were seropositive and 89.4% (42/47) of the IgG positives had neutralising antibodies. Most of the symptomatic individuals confirmed as polymerase chain reaction (PCR) positive during the outbreak were seropositive (30/32, 93.8%) and 33.3% of the individuals who quarantined with a PCR confirmed patient had antibodies. Serological assays comparison revealed that Architect (Abbott) targeting the N protein LIASON® (DiaSorin) targeting the S protein and enzyme-linked immunosorbent assay (ELISA) targeting receptor binding domain detected 9.5% (27/283), 17.3% (49/283) and 17% (48/283), respectively, as IgG positives. The latter two assays highly agreed (kappa = 0.89) between each other. In addition, 95%, (19/20, by ELISA) and 90.9% (20/22, with LIASON) and only 71.4% (15/21, by Architect) of individuals that were seropositive in May 2020 were found positive also in September. The unexpected low rate of overall immunity indicates the absence of un-noticed, asymptomatic infections. Lack of overall high correlation between the assays is attributed mainly to target-mediated antibody responses and suggests that using a single serological assay may be misleading.
Keywords: Herd immunity; IgG antibodies; SARS-CoV-2; neutralising antibodies; sero-prevalence.