tetano
Editor, Senior Moderator
Bing Du Xue Bao. 2012 Jun;28(4):324-9.
[Establishing a high-titer infectious avian influenza A (H5N1) pseudotyped viral particle].
[Article in Chinese]
Liu XJ, Guo ZP, Shen LP, Wang Y, Shi HY, Zhang GH, Zhang X, Bi SL, Zhao HL.
Source
Institute of Medical Virology, School of Laboratory Medicine and Life Science, Wenzhou Medical College, Wenzhou 325035, China. liuxijun.jack@126.com
Abstract
A transient four-plasmid cotransfection system was used to construct avian influenza A (H5N1) pseudotyped viral particle (H5N1Pp) by incorporating hemagglutinin (HA) protein and neuraminidase (NA) protein from H5N1 avian influenza virus onto Murine leukemia virus pseudotyped viral particles, the transmission electron microscopy, infectivity titer assay, hemagglutination assay, neutralization assay of H5N1Pp were studied. We established a pseudotyped H5N1 viral particle at a high titer of 10(8) Pp/mL, the morphology,the hemagglutination activity and neutralization specificity of H5N1Pp is simililar to wild H5N1 virus. The research result sets a platform for studying this virus, including its receptors, the functional analysis of HA and NA, neutralizing antibodies and anti-H5N1 drug development.
PMID:
22978154
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/22978154
[Establishing a high-titer infectious avian influenza A (H5N1) pseudotyped viral particle].
[Article in Chinese]
Liu XJ, Guo ZP, Shen LP, Wang Y, Shi HY, Zhang GH, Zhang X, Bi SL, Zhao HL.
Source
Institute of Medical Virology, School of Laboratory Medicine and Life Science, Wenzhou Medical College, Wenzhou 325035, China. liuxijun.jack@126.com
Abstract
A transient four-plasmid cotransfection system was used to construct avian influenza A (H5N1) pseudotyped viral particle (H5N1Pp) by incorporating hemagglutinin (HA) protein and neuraminidase (NA) protein from H5N1 avian influenza virus onto Murine leukemia virus pseudotyped viral particles, the transmission electron microscopy, infectivity titer assay, hemagglutination assay, neutralization assay of H5N1Pp were studied. We established a pseudotyped H5N1 viral particle at a high titer of 10(8) Pp/mL, the morphology,the hemagglutination activity and neutralization specificity of H5N1Pp is simililar to wild H5N1 virus. The research result sets a platform for studying this virus, including its receptors, the functional analysis of HA and NA, neutralizing antibodies and anti-H5N1 drug development.
PMID:
22978154
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/22978154