tetano
Editor, Senior Moderator
Eur J Immunol
. 2020 Dec 12.
doi: 10.1002/eji.202048978. Online ahead of print.
Type-I Interferon assessment in 45 minutes using the FilmArray [SUP]?[/SUP] PCR platform in SARS-CoV-2 and other viral infections
Marine Mommert[SUP] 1 2 [/SUP], Magali Perret[SUP] 3 4 [/SUP], Matthew Hockin[SUP] 5 [/SUP], S?bastien Viel[SUP] 3 4 6 7 [/SUP], Alexandre Belot[SUP] 8 [/SUP], Jean-Christophe Richard[SUP] 9 [/SUP], Mehdi Mezidi[SUP] 9 [/SUP], Jean-Baptiste Fassier[SUP] 10 [/SUP], Etienne Javouhey[SUP] 11 [/SUP], ANTOINE Study group; COVID-SER Study group; COVID-HCL Study group; Andrew Hemmert[SUP] 5 [/SUP], Fran?ois Mallet[SUP] 1 2 [/SUP], Sophie Trouillet-Assant[SUP] 1 3 [/SUP], Karen Brengel-Pesce[SUP] 1 2 [/SUP]
Affiliations
Abstract
Low concentrations of Type-I interferon (IFN) in blood seems to be associated with more severe forms of Coronavirus disease 2019 (COVID-19). However, following the type-I interferon response (IR) in early stage disease is a major challenge. We evaluated detection of a molecular interferon signature on a FilmArray? system, which includes PCR assays for four interferon stimulated genes. We analysed 3 types of patient populations: i) children admitted to a pediatric emergency unit for fever and suspected infection, ii) ICU-admitted patients with severe COVID-19 and iii) healthcare workers with mild COVID-19. The results were compared to the reference tools, i.e. molecular signature assessed with Nanostring? and IFN-α2 quantification by SIMOA? (Single MOlecule Array). A strong correlation was observed between the IR measured by the FilmArray?, Nanostring?, and SIMOA? platforms (r-Spearman 0.996 and 0.838, respectively). The FilmArray? panel could be used in the COVID-19 pandemic to evaluate the IR in 45-minutes with 2 minutes hand-on-time at hospitalization and to monitor the IR in future clinical trials. This article is protected by copyright. All rights reserved.
Keywords: COVID-19; FilmArray?; Interferon; clinical application; in vitro diagnostic.
. 2020 Dec 12.
doi: 10.1002/eji.202048978. Online ahead of print.
Type-I Interferon assessment in 45 minutes using the FilmArray [SUP]?[/SUP] PCR platform in SARS-CoV-2 and other viral infections
Marine Mommert[SUP] 1 2 [/SUP], Magali Perret[SUP] 3 4 [/SUP], Matthew Hockin[SUP] 5 [/SUP], S?bastien Viel[SUP] 3 4 6 7 [/SUP], Alexandre Belot[SUP] 8 [/SUP], Jean-Christophe Richard[SUP] 9 [/SUP], Mehdi Mezidi[SUP] 9 [/SUP], Jean-Baptiste Fassier[SUP] 10 [/SUP], Etienne Javouhey[SUP] 11 [/SUP], ANTOINE Study group; COVID-SER Study group; COVID-HCL Study group; Andrew Hemmert[SUP] 5 [/SUP], Fran?ois Mallet[SUP] 1 2 [/SUP], Sophie Trouillet-Assant[SUP] 1 3 [/SUP], Karen Brengel-Pesce[SUP] 1 2 [/SUP]
Affiliations
- PMID: 33314090
- DOI: 10.1002/eji.202048978
Abstract
Low concentrations of Type-I interferon (IFN) in blood seems to be associated with more severe forms of Coronavirus disease 2019 (COVID-19). However, following the type-I interferon response (IR) in early stage disease is a major challenge. We evaluated detection of a molecular interferon signature on a FilmArray? system, which includes PCR assays for four interferon stimulated genes. We analysed 3 types of patient populations: i) children admitted to a pediatric emergency unit for fever and suspected infection, ii) ICU-admitted patients with severe COVID-19 and iii) healthcare workers with mild COVID-19. The results were compared to the reference tools, i.e. molecular signature assessed with Nanostring? and IFN-α2 quantification by SIMOA? (Single MOlecule Array). A strong correlation was observed between the IR measured by the FilmArray?, Nanostring?, and SIMOA? platforms (r-Spearman 0.996 and 0.838, respectively). The FilmArray? panel could be used in the COVID-19 pandemic to evaluate the IR in 45-minutes with 2 minutes hand-on-time at hospitalization and to monitor the IR in future clinical trials. This article is protected by copyright. All rights reserved.
Keywords: COVID-19; FilmArray?; Interferon; clinical application; in vitro diagnostic.