Giuseppe
Emeritus
Guidelines for the laboratory diagnosis of genital herpes in eastern European countries (Euro Surveill., abstract, edited)
[Source: Eurosurveillance, full text: <cite cite="http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=19703">Eurosurveillance - View Article</cite>. Abstract, edited.]
Eurosurveillance, Volume 15, Issue 44, 04 November 2010
Perspectives
Guidelines for the laboratory diagnosis of genital herpes in eastern European countries
M Domeika 1, M Bashmakova 2, A Savicheva 2, N Kolomiec 3, E Sokolovskiy 4, A Hall?n 5, M Unemo 6, R C Ballard 7, Eastern European Network for Sexual and Reproductive Health (EE SRH Network) 8
1. Department of Medical Sciences, Uppsala University, Uppsala, Sweden
2. Microbiology Laboratory, DO Ott Institute for Obstetrics and Gynaecology, St Petersburg, Russia
3. Microbiology Department, Belarus Medical Academy of Postgraduate Studies, Minsk, Belarus
4. Department of Dermatology and Venereology, Pavlov State Medical University, St Petersburg, Russia
5. Department of Dermatology and Venereology, Uppsala University Hospital, Uppsala, Sweden
6. Department of Laboratory Medicine, Clinical Microbiology, ?rebro University Hospital, ?rebro, Sweden
7. National Center for HIV, STD, and TB prevention, Centers for Disease Control and Prevention (CDC), Atlanta, United States
8. Member of the network are listed at the end of the article
Citation style for this article: Domeika M, Bashmakova M, Savicheva A, Kolomiec N, Sokolovskiy E, Hall?n A, Unemo M, Ballard RC, Eastern European Network for Sexual and Reproductive Health (EE SRH Network). Guidelines for the laboratory diagnosis of genital herpes in eastern European countries. Euro Surveill. 2010;15(44)
ii=19703. Available online: http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=19703
Date of submission: 04 November 2009
These guidelines aim to provide comprehensive information about sexually transmitted herpes simplex virus (HSV) infection and its laboratory diagnosis in eastern European countries. They are primarily intended for professionals testing specimens from patients at a sexual healthcare clinic but may also be helpful for community-based screening programmes. In particular, the guidelines recommend: (i) either viral culture or validated and approved nucleic acid amplification tests (NAATs) as the tests of choice for symptomatic patients, which should be promoted for laboratory confirmation of HSV infection; (ii) if culture or NAATs are not available, antigen detection ? a direct immunofluorescence test or enzyme immunoassay from samples from symptomatic patients ? could be employed, but HSV type determination is of importance; (iii) only type-specific serology should be used for detecting asymptomatic individuals, testing pregnant women at risk of acquiring HSV infection close to delivery, men who have sex with men and people who are HIV positive; (iv) widespread screening for HSV antibodies should be discouraged; and (v) any non-validated diagnostic tests should be validated against a recommended, approved gold standard.
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[Source: Eurosurveillance, full text: <cite cite="http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=19703">Eurosurveillance - View Article</cite>. Abstract, edited.]
Eurosurveillance, Volume 15, Issue 44, 04 November 2010
Perspectives
Guidelines for the laboratory diagnosis of genital herpes in eastern European countries
M Domeika 1, M Bashmakova 2, A Savicheva 2, N Kolomiec 3, E Sokolovskiy 4, A Hall?n 5, M Unemo 6, R C Ballard 7, Eastern European Network for Sexual and Reproductive Health (EE SRH Network) 8
1. Department of Medical Sciences, Uppsala University, Uppsala, Sweden
2. Microbiology Laboratory, DO Ott Institute for Obstetrics and Gynaecology, St Petersburg, Russia
3. Microbiology Department, Belarus Medical Academy of Postgraduate Studies, Minsk, Belarus
4. Department of Dermatology and Venereology, Pavlov State Medical University, St Petersburg, Russia
5. Department of Dermatology and Venereology, Uppsala University Hospital, Uppsala, Sweden
6. Department of Laboratory Medicine, Clinical Microbiology, ?rebro University Hospital, ?rebro, Sweden
7. National Center for HIV, STD, and TB prevention, Centers for Disease Control and Prevention (CDC), Atlanta, United States
8. Member of the network are listed at the end of the article
Citation style for this article: Domeika M, Bashmakova M, Savicheva A, Kolomiec N, Sokolovskiy E, Hall?n A, Unemo M, Ballard RC, Eastern European Network for Sexual and Reproductive Health (EE SRH Network). Guidelines for the laboratory diagnosis of genital herpes in eastern European countries. Euro Surveill. 2010;15(44)
ii=19703. Available online: http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=19703Date of submission: 04 November 2009
These guidelines aim to provide comprehensive information about sexually transmitted herpes simplex virus (HSV) infection and its laboratory diagnosis in eastern European countries. They are primarily intended for professionals testing specimens from patients at a sexual healthcare clinic but may also be helpful for community-based screening programmes. In particular, the guidelines recommend: (i) either viral culture or validated and approved nucleic acid amplification tests (NAATs) as the tests of choice for symptomatic patients, which should be promoted for laboratory confirmation of HSV infection; (ii) if culture or NAATs are not available, antigen detection ? a direct immunofluorescence test or enzyme immunoassay from samples from symptomatic patients ? could be employed, but HSV type determination is of importance; (iii) only type-specific serology should be used for detecting asymptomatic individuals, testing pregnant women at risk of acquiring HSV infection close to delivery, men who have sex with men and people who are HIV positive; (iv) widespread screening for HSV antibodies should be discouraged; and (v) any non-validated diagnostic tests should be validated against a recommended, approved gold standard.
-
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