Eurosurv. SARS-CoV-2 neutralising antibody testing in Europe: towards harmonisation of neutralising antibody titres for better use of convalescent pla

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SARS-CoV-2 neutralising antibody testing in Europe: towards harmonisation of neutralising antibody titres for better use of convalescent plasma and comparability of trial data

• Dung Nguyen[SUP]1[/SUP] , Peter Simmonds[SUP]1[/SUP] , Maurice Steenhuis[SUP]2[/SUP] , Elise Wouters[SUP]3[/SUP] , Daniel Desmecht[SUP]4[/SUP] , Mutien Garigliany[SUP]4[/SUP] , Marta Romano[SUP]5[/SUP] , Cyril Barbezange[SUP]6[/SUP] , Piet Maes[SUP]7[/SUP] , Bram Van Holm[SUP]7[/SUP] , Joaquín Mendoza[SUP]8[/SUP] , Salvador Oyonarte[SUP]9[/SUP] , Anders Fomsgaard[SUP]10[/SUP] , Ria Lassaunière[SUP]10[/SUP] , Eva Zusinaite[SUP]11[/SUP] , Katarina Resman Rus[SUP]12[/SUP] , Tatjana Avšič-Županc[SUP]12[/SUP] , Johan HJ Reimerink[SUP]13[/SUP] , Fiona Brouwer[SUP]13[/SUP] , Marieke Hoogerwerf[SUP]13[/SUP] , Chantal BEM Reusken[SUP]13[/SUP] , Gunnveig Grodeland[SUP]14[/SUP] , Sophie Le Cam[SUP]15[/SUP] , Pierre Gallian[SUP]15,16[/SUP] , Abdennour Amroun[SUP]16[/SUP] , Nadège Brisbarre[SUP]16,17[/SUP] , Christophe Martinaud[SUP]18[/SUP] , Isabelle Leparc Goffart[SUP]19[/SUP] , Hubert Schrezenmeier[SUP]20,21[/SUP] , Hendrik B Feys[SUP]3[/SUP] , C Ellen van der Schoot[SUP]2[/SUP] , Heli Harvala[SUP]22,23[/SUP]

We compared the performance of SARS-CoV-2 neutralising antibody testing between 12 European laboratories involved in convalescent plasma trials. Raw titres differed almost 100-fold differences between laboratories when blind-testing 15 plasma samples. Calibration of titres in relation to the reference reagent and standard curve obtained by testing a dilution series reduced the inter-laboratory variability ca 10-fold. The harmonisation of neutralising antibody quantification is a vital step towards determining the protective and therapeutic levels of neutralising antibodies.

full article

https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2021.26.27.2100568#html_fulltext