tetano
Editor, Senior Moderator
Influenza Other Respir Viruses. 2013 Nov 24. doi: 10.1111/irv.12210. [Epub ahead of print]
Evaluating the performance of a rapid antigen test for the detection of influenza virus in clinical specimens from children in Cameroon.
Kenmoe S, Tchendjou P, Moyo Tetang S, Mossus T, Njankouo Ripa M, Guillet M, Kfutwah A, Njouom R.
Source
National Influenza Centre, Centre Pasteur of Cameroon, Yaounde, Cameroon; Universit? de Yaound? I, Yaounde, Cameroon.
Abstract
The performance of SD Bioline rapid antigen test (RAT) was evaluated using real-time reverse transcription polymerase chain reaction (rRT-PCR) as gold standard. A total of 718 nasal swabs, including 102 rRT-PCR positive and 616 rRT-PCR negative swabs, were tested. RAT demonstrates a sensitivity of 29?4% with a specificity of 100%. The positivity rate of RAT was highly associated with lower cycle threshold (Ct) values (P < 0?0001). The excellent specificity of the RAT allowed for the rapid identification of influenza cases. However, negative results should be verified by rRT-PCR test because of limitations observed in sensitivity.
? 2013 The Authors. Influenza and Other Respiratory Viruses published by John Wiley & Sons Ltd.
KEYWORDS:
Cameroon, SD Bioline, influenza, sensitivity, specificity
PMID:
24266902
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24266902
Evaluating the performance of a rapid antigen test for the detection of influenza virus in clinical specimens from children in Cameroon.
Kenmoe S, Tchendjou P, Moyo Tetang S, Mossus T, Njankouo Ripa M, Guillet M, Kfutwah A, Njouom R.
Source
National Influenza Centre, Centre Pasteur of Cameroon, Yaounde, Cameroon; Universit? de Yaound? I, Yaounde, Cameroon.
Abstract
The performance of SD Bioline rapid antigen test (RAT) was evaluated using real-time reverse transcription polymerase chain reaction (rRT-PCR) as gold standard. A total of 718 nasal swabs, including 102 rRT-PCR positive and 616 rRT-PCR negative swabs, were tested. RAT demonstrates a sensitivity of 29?4% with a specificity of 100%. The positivity rate of RAT was highly associated with lower cycle threshold (Ct) values (P < 0?0001). The excellent specificity of the RAT allowed for the rapid identification of influenza cases. However, negative results should be verified by rRT-PCR test because of limitations observed in sensitivity.
? 2013 The Authors. Influenza and Other Respiratory Viruses published by John Wiley & Sons Ltd.
KEYWORDS:
Cameroon, SD Bioline, influenza, sensitivity, specificity
PMID:
24266902
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24266902