tetano
Editor, Senior Moderator
J Virol Methods. 2015 Nov 29. pii: S0166-0934(15)00382-1. doi: 10.1016/j.jviromet.2015.11.021. [Epub ahead of print]
[h=1]Evaluation and optimization of a commercial influenza A blocking ELISA for research and surveillance of mallards.[/h] Shriner SA[SUP]1[/SUP], VanDalen KK[SUP]2[/SUP], Root JJ[SUP]2[/SUP], Sullivan HJ[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The availability of a validated commercial assay is an asset for any wildlife investigation. However, commercial products are often developed for use in livestock and are not optimized for wildlife. Consequently, it is incumbent upon researchers and managers to apply commercial products appropriately to optimize program outcomes. We tested more than 800 serum samples from mallards for antibodies to influenza A with the IDEXX AI MultiS-Screen Ab test to evaluate assay performance. Applying the test per manufacturer's recommendations resulted in good performance with 84% sensitivity and 100% specificity. However, performance was improved to 98% sensitivity and 98% specificity by increasing the recommended cut-off. Using this alternative threshold for identifying positive and negative samples would greatly improve sample classification, especially for field samples collected months after infection when antibody titers have waned from the initial primary immune response. Furthermore, a threshold that balances sensitivity and specificity reduces estimation bias in seroprevlanece estimates.
Copyright ? 2015. Published by Elsevier B.V.
PMID: 26645952 [PubMed - as supplied by publisher]
[h=1]Evaluation and optimization of a commercial influenza A blocking ELISA for research and surveillance of mallards.[/h] Shriner SA[SUP]1[/SUP], VanDalen KK[SUP]2[/SUP], Root JJ[SUP]2[/SUP], Sullivan HJ[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The availability of a validated commercial assay is an asset for any wildlife investigation. However, commercial products are often developed for use in livestock and are not optimized for wildlife. Consequently, it is incumbent upon researchers and managers to apply commercial products appropriately to optimize program outcomes. We tested more than 800 serum samples from mallards for antibodies to influenza A with the IDEXX AI MultiS-Screen Ab test to evaluate assay performance. Applying the test per manufacturer's recommendations resulted in good performance with 84% sensitivity and 100% specificity. However, performance was improved to 98% sensitivity and 98% specificity by increasing the recommended cut-off. Using this alternative threshold for identifying positive and negative samples would greatly improve sample classification, especially for field samples collected months after infection when antibody titers have waned from the initial primary immune response. Furthermore, a threshold that balances sensitivity and specificity reduces estimation bias in seroprevlanece estimates.
Copyright ? 2015. Published by Elsevier B.V.
PMID: 26645952 [PubMed - as supplied by publisher]