tetano
Editor, Senior Moderator
Fetal Pediatr Pathol. 2019 Jul 15:1-6. doi: 10.1080/15513815.2019.1639088. [Epub ahead of print]
[h=1]Evaluation of a Commercial Colloidal Gold Assay for Detection of Influenza A and B Virus in Children's Respiratory Specimens.[/h] Li W[SUP]1[/SUP], Liu L[SUP]2[/SUP], Chen L[SUP]2[/SUP], Shang S[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Objective: To evaluate the clinical diagnostic value of colloidal gold assay for detection of influenza A and B virus. Methods: Results were compared for colloidal gold assay for influenza antigen detection and real-time RT-PCR for RNA detection. Results: By performing the colloidal gold assay, the positive rate was 25.67% (105/409) for influenza A virus and 8.56% (35/409) for influenza B virus. While tested by real-time RT-PCR, 107(26.2%) were positive for influenza A virus and 35(8.6%) for influenza B virus. Using real-time RT-PCR as the gold standard, the sensitivity and specificity of the colloidal gold assay were 84.1% and 95.0%, respectively, for influenza A virus and 85.7% and 98.7% for influenza B virus. Conclusion: This colloidal gold assay may be one of the viable tools for the rapid screening for influenza viruses in an outpatient clinical practice.
[h=4]KEYWORDS:[/h] Influenza virus; colloidal gold assay; real-time RT-PCR
PMID: 31304835 DOI: 10.1080/15513815.2019.1639088
[h=1]Evaluation of a Commercial Colloidal Gold Assay for Detection of Influenza A and B Virus in Children's Respiratory Specimens.[/h] Li W[SUP]1[/SUP], Liu L[SUP]2[/SUP], Chen L[SUP]2[/SUP], Shang S[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Objective: To evaluate the clinical diagnostic value of colloidal gold assay for detection of influenza A and B virus. Methods: Results were compared for colloidal gold assay for influenza antigen detection and real-time RT-PCR for RNA detection. Results: By performing the colloidal gold assay, the positive rate was 25.67% (105/409) for influenza A virus and 8.56% (35/409) for influenza B virus. While tested by real-time RT-PCR, 107(26.2%) were positive for influenza A virus and 35(8.6%) for influenza B virus. Using real-time RT-PCR as the gold standard, the sensitivity and specificity of the colloidal gold assay were 84.1% and 95.0%, respectively, for influenza A virus and 85.7% and 98.7% for influenza B virus. Conclusion: This colloidal gold assay may be one of the viable tools for the rapid screening for influenza viruses in an outpatient clinical practice.
[h=4]KEYWORDS:[/h] Influenza virus; colloidal gold assay; real-time RT-PCR
PMID: 31304835 DOI: 10.1080/15513815.2019.1639088