tetano
Editor, Senior Moderator
J Clin Microbiol. 2014 Jul 2. pii: JCM.01132-14. [Epub ahead of print]
Evaluation of Alere i Influenza A&B for Rapid Detection of Influenza Viruses A and B.
Nie S1, Roth R2, Stiles J3, Mikhlina A3, Lu X4, Tang YW5, Babady NE3.
Author information
Abstract
Rapid and accurate diagnosis of influenza (Flu) is important for infection control as well as for patient management. Alere i Influenza A&B is an isothermal nucleic acid amplification-based integrated system for detection and differentiation of FluA and FluB. The performance of the Alere i Influenza A&B was screened using frozen nasopharyngeal swab specimens collected in viral transport media (VTM) that were originally tested fresh with the FilmArray Respiratory Panel (RP) assay during the 2012/2013 influenza outbreak. In total, 360 VTM specimens were selected for Alere i Influenza A&B testing, including 40 FluA H1N1-2009 (FluA-1), 40 FluA H3N2 (FluA-3), 37 FluA "equivocal" or "no subtype detected" (FluA-u), 41 FluB and 202 Flu-negatives as initially determined by the FilmArray RP assay. The Alere assay showed sensitivities of 87.2%, 92.5%, 25.0% and 97.4% for FluA-1, FluA-3, FluA-u, and FluB, respectively, after discordant resolution by Prodesse ProFLU+ PCR. The specificities were 100% for both FluA and FluB. In general, the Alere i Influenza A&B provided good sensitivity although the assay did show poorer sensitivity with samples determined to be of low FluA titer by Prodesse ProFlu+ PCR (mean real-time PCR threshold cycle (CT) value of 31.9 ? 2.0), which included the majority of the samples called FluA "equivocal" or "no subtype detected" by a single BioFire FilmArray RP test. The integrated, rapid and simple characteristics of the Alere i Influenza A&B make it potential for point-of-care testing with a test turnaround time less than 15 minutes.
Copyright ? 2014, American Society for Microbiology. All Rights Reserved.
PMID:
24989611
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24989611
Evaluation of Alere i Influenza A&B for Rapid Detection of Influenza Viruses A and B.
Nie S1, Roth R2, Stiles J3, Mikhlina A3, Lu X4, Tang YW5, Babady NE3.
Author information
Abstract
Rapid and accurate diagnosis of influenza (Flu) is important for infection control as well as for patient management. Alere i Influenza A&B is an isothermal nucleic acid amplification-based integrated system for detection and differentiation of FluA and FluB. The performance of the Alere i Influenza A&B was screened using frozen nasopharyngeal swab specimens collected in viral transport media (VTM) that were originally tested fresh with the FilmArray Respiratory Panel (RP) assay during the 2012/2013 influenza outbreak. In total, 360 VTM specimens were selected for Alere i Influenza A&B testing, including 40 FluA H1N1-2009 (FluA-1), 40 FluA H3N2 (FluA-3), 37 FluA "equivocal" or "no subtype detected" (FluA-u), 41 FluB and 202 Flu-negatives as initially determined by the FilmArray RP assay. The Alere assay showed sensitivities of 87.2%, 92.5%, 25.0% and 97.4% for FluA-1, FluA-3, FluA-u, and FluB, respectively, after discordant resolution by Prodesse ProFLU+ PCR. The specificities were 100% for both FluA and FluB. In general, the Alere i Influenza A&B provided good sensitivity although the assay did show poorer sensitivity with samples determined to be of low FluA titer by Prodesse ProFlu+ PCR (mean real-time PCR threshold cycle (CT) value of 31.9 ? 2.0), which included the majority of the samples called FluA "equivocal" or "no subtype detected" by a single BioFire FilmArray RP test. The integrated, rapid and simple characteristics of the Alere i Influenza A&B make it potential for point-of-care testing with a test turnaround time less than 15 minutes.
Copyright ? 2014, American Society for Microbiology. All Rights Reserved.
PMID:
24989611
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24989611