tetano
Editor, Senior Moderator
J Infect Chemother. 2017 Aug 19. pii: S1341-321X(17)30177-0. doi: 10.1016/j.jiac.2017.07.013. [Epub ahead of print]
[h=1]Evaluation of Anyplex? II RV16 and RB5 real-time RT-PCR compared to Seeplex? RV15 OneStep ACE and PneumoBacter ACE for the simultaneous detection of upper respiratory pathogens.[/h] Parrott G[SUP]1[/SUP], Kinjo T[SUP]2[/SUP], Nabeya D[SUP]2[/SUP], Uehara A[SUP]2[/SUP], Nahar S[SUP]2[/SUP], Miyagi K[SUP]2[/SUP], Haranaga S[SUP]2[/SUP], Tateyama M[SUP]2[/SUP], Fujita J[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] This prospective study was performed to evaluate and compare the performance of the multiplex PCR Seeplex[SUP]?[/SUP] assays and Anyplex? II assays. From May 2014 until April 2016, a total of 247 respiratory samples were collected in Okinawa, Japan. Multiple respiratory pathogens were detected in 37% of patients with positive results. The most prevalent pathogens were influenza A virus and respiratory syncytial virus B. Despite minor differences in capabilities, both the Seeplex[SUP]?[/SUP] assays and Anyplex? II assays can be easily implemented in diagnostic or research laboratories to optimize the detection and management of respiratory pathogen induced diseases.
Copyright ? 2017 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
[h=4]KEYWORDS:[/h] Molecular diagnostics; Multiplex PCR; Upper respiratory infections
PMID: 28830668 DOI: 10.1016/j.jiac.2017.07.013
[h=1]Evaluation of Anyplex? II RV16 and RB5 real-time RT-PCR compared to Seeplex? RV15 OneStep ACE and PneumoBacter ACE for the simultaneous detection of upper respiratory pathogens.[/h] Parrott G[SUP]1[/SUP], Kinjo T[SUP]2[/SUP], Nabeya D[SUP]2[/SUP], Uehara A[SUP]2[/SUP], Nahar S[SUP]2[/SUP], Miyagi K[SUP]2[/SUP], Haranaga S[SUP]2[/SUP], Tateyama M[SUP]2[/SUP], Fujita J[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] This prospective study was performed to evaluate and compare the performance of the multiplex PCR Seeplex[SUP]?[/SUP] assays and Anyplex? II assays. From May 2014 until April 2016, a total of 247 respiratory samples were collected in Okinawa, Japan. Multiple respiratory pathogens were detected in 37% of patients with positive results. The most prevalent pathogens were influenza A virus and respiratory syncytial virus B. Despite minor differences in capabilities, both the Seeplex[SUP]?[/SUP] assays and Anyplex? II assays can be easily implemented in diagnostic or research laboratories to optimize the detection and management of respiratory pathogen induced diseases.
Copyright ? 2017 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
[h=4]KEYWORDS:[/h] Molecular diagnostics; Multiplex PCR; Upper respiratory infections
PMID: 28830668 DOI: 10.1016/j.jiac.2017.07.013