tetano
Editor, Senior Moderator
Avian Dis. 2011 Dec;55(4):674-6.
Evaluation of Nobuto filter paper strips for the detection of avian influenza virus antibody in waterfowl.
Dusek RJ, Hall JS, Nashold SW, TeSlaa JL, Ip HS.
Source
United States Geological Survey, National Wildlife Health Center, Madison, WI 53711, USA. rdusek@usgs.gov
Abstract
The utility of using Nobuto paper strips for the detection of avian influenza antibodies was examined in mallards (Anas platyrhynchos) experimentally infected with low pathogenic avian influenza viruses. Blood was collected 2 wk after infection and was preserved either as serum or whole blood absorbed onto Nobuto strips. Analysis of samples using a commercially available blocking enzyme-linked immunosorbent assay revealed comparable results (> or = 96% sensitivity for all methods) between sera stored at -30 C and the Nobuto strip preservation method even when the Nobuto strips were stored up to 3 mo at room temperature (RT). Significant differences were detected in the ratio of sample absorbance to negative control absorbance for Nobuto strips stored at RT compared with sera stored at -30 C, although these differences did not affect the ability of the test to reliably detect positive and negative samples. Nobuto strips are a convenient and sensitive alternative to the collection of serum samples when maintaining appropriate storage temperatures is difficult.
PMID:
22312989
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/22312989
Evaluation of Nobuto filter paper strips for the detection of avian influenza virus antibody in waterfowl.
Dusek RJ, Hall JS, Nashold SW, TeSlaa JL, Ip HS.
Source
United States Geological Survey, National Wildlife Health Center, Madison, WI 53711, USA. rdusek@usgs.gov
Abstract
The utility of using Nobuto paper strips for the detection of avian influenza antibodies was examined in mallards (Anas platyrhynchos) experimentally infected with low pathogenic avian influenza viruses. Blood was collected 2 wk after infection and was preserved either as serum or whole blood absorbed onto Nobuto strips. Analysis of samples using a commercially available blocking enzyme-linked immunosorbent assay revealed comparable results (> or = 96% sensitivity for all methods) between sera stored at -30 C and the Nobuto strip preservation method even when the Nobuto strips were stored up to 3 mo at room temperature (RT). Significant differences were detected in the ratio of sample absorbance to negative control absorbance for Nobuto strips stored at RT compared with sera stored at -30 C, although these differences did not affect the ability of the test to reliably detect positive and negative samples. Nobuto strips are a convenient and sensitive alternative to the collection of serum samples when maintaining appropriate storage temperatures is difficult.
PMID:
22312989
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/22312989