tetano
Editor, Senior Moderator
Vet Immunol Immunopathol. 2017 Mar;185:57-65. doi: 10.1016/j.vetimm.2017.01.009. Epub 2017 Feb 5.
[h=1]Factors affecting induction of peripheral IFN-γ recall response to influenza A virus vaccination in pigs.[/h] Olson ZF[SUP]1[/SUP], Sandbulte MR[SUP]2[/SUP], Souza CK[SUP]1[/SUP], Perez DR[SUP]3[/SUP], Vincent AL[SUP]1[/SUP], Loving CL[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] While T cell contribution to IAV immunity is appreciated, data comparing methods to evaluate IFN-γ production by IAV-specific T cells elicited following vaccination is limited. To understand the differential immunogenicity between live-attenuated influenza virus (LAIV) and whole-inactivated virus (WIV) vaccines in relation to induction of peripheral T cell responses, ELISpot and ELISA were used to assess IFN-γ production by peripheral lymphocytes following antigen restimulation. Following restimulation, peripheral blood lymphocytes from WIV-vaccinated pigs had a greater quantity of IFN-γ secreting cells (SC) and IFN-γ secreted compared to LAIV vaccinated and non-vaccinated (NV) pigs. Pig age at time of WIV vaccination significantly impacted peripheral IAV-specific IFN-γ recall response, as did the inclusion of adjuvant in the WIV vaccine. Collectively, these data indicate that peripheral IAV-specific IFN-γ recall responses are not predictive of LAIV vaccination status, thus, are unlikely to be a useful surrogate for evaluating LAIV immunogenicity and predicting cross-protection. However, these data suggest that the evaluation of peripheral IFN-γ recall responses may be useful for identifying factors, such as animal age or vaccine formulation, that may impact parenterally-delivered WIV vaccine immunogenicity. Overall, results did not differ based upon the assay used to evaluate IFN-γ recall responses. Therefore, either ELISpot or ELISA could serve as a measure for evaluating IAV-specific IFN-γ cell-mediated immune responses in swine.
Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] Cell-mediated immunity; IFN-gamma; Immune; Influenza; Porcine; Vaccine
PMID: 28242003 DOI: 10.1016/j.vetimm.2017.01.009
[PubMed - in process]
[h=1]Factors affecting induction of peripheral IFN-γ recall response to influenza A virus vaccination in pigs.[/h] Olson ZF[SUP]1[/SUP], Sandbulte MR[SUP]2[/SUP], Souza CK[SUP]1[/SUP], Perez DR[SUP]3[/SUP], Vincent AL[SUP]1[/SUP], Loving CL[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] While T cell contribution to IAV immunity is appreciated, data comparing methods to evaluate IFN-γ production by IAV-specific T cells elicited following vaccination is limited. To understand the differential immunogenicity between live-attenuated influenza virus (LAIV) and whole-inactivated virus (WIV) vaccines in relation to induction of peripheral T cell responses, ELISpot and ELISA were used to assess IFN-γ production by peripheral lymphocytes following antigen restimulation. Following restimulation, peripheral blood lymphocytes from WIV-vaccinated pigs had a greater quantity of IFN-γ secreting cells (SC) and IFN-γ secreted compared to LAIV vaccinated and non-vaccinated (NV) pigs. Pig age at time of WIV vaccination significantly impacted peripheral IAV-specific IFN-γ recall response, as did the inclusion of adjuvant in the WIV vaccine. Collectively, these data indicate that peripheral IAV-specific IFN-γ recall responses are not predictive of LAIV vaccination status, thus, are unlikely to be a useful surrogate for evaluating LAIV immunogenicity and predicting cross-protection. However, these data suggest that the evaluation of peripheral IFN-γ recall responses may be useful for identifying factors, such as animal age or vaccine formulation, that may impact parenterally-delivered WIV vaccine immunogenicity. Overall, results did not differ based upon the assay used to evaluate IFN-γ recall responses. Therefore, either ELISpot or ELISA could serve as a measure for evaluating IAV-specific IFN-γ cell-mediated immune responses in swine.
Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] Cell-mediated immunity; IFN-gamma; Immune; Influenza; Porcine; Vaccine
PMID: 28242003 DOI: 10.1016/j.vetimm.2017.01.009
[PubMed - in process]