tetano
Editor, Senior Moderator
Immunology. 2014 Apr 28. doi: 10.1111/imm.12310. [Epub ahead of print]
Fc receptor is not required for inducing antibodies but plays a critical role in conferring protection after influenza M2 vaccination.
Lee YN1, Lee YT, Kim MC, Hwang HS, Lee JS, Kim KH, Kang SM.
Author information
Abstract
The ectodomain of matrix protein 2 (M2e) of influenza virus is considered a rational target for a universal influenza A vaccine. To better understand M2e immune-mediated protection, Fc receptor common γ chain deficient (FcR γ-/- ) and wild type mice were immunized with a tandem repeat of M2e presented on virus-like particles (M2e5x VLP). Levels of M2e-specific antibodies that were induced in FcR γ-/- mice after immunization with M2e5x VLP were similar to those in wild type mice. In addition, M2e antibodies induced in FcR γ-/- mice were found to be equally protective as compared to those induced in wild type mice. However, M2e5x VLP-immunized FcR γ-/- mice were not well protected as evidenced by severe weight loss, higher lung viral titers, and IL-6 inflammatory cytokine production upon influenza virus challenge infection compared to M2e5x VLP-immunized wild type mice. Importantly, FcR γ-/- mice that were immunized with inactivated influenza virus induced hemagglutination inhibition activity and were well protected without a significant weight loss. Interestingly, interferon-γ producing CD4 T and CD8 T cells were found to be high in lungs from M2e5x VLP immunized FcR γ-/- mice, which appeared to be correlated with a faster recovery after infection. These results indicate that Fc receptors play a primary role in conferring M2e specific antibody-mediated protection whereas T cells may contribute to the recovery at later stages of infection. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
KEYWORDS:
Fc receptor, Influenza virus, M2e VLPs, M2e antibodies, T cells
PMID:
24773389
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24773389
Fc receptor is not required for inducing antibodies but plays a critical role in conferring protection after influenza M2 vaccination.
Lee YN1, Lee YT, Kim MC, Hwang HS, Lee JS, Kim KH, Kang SM.
Author information
Abstract
The ectodomain of matrix protein 2 (M2e) of influenza virus is considered a rational target for a universal influenza A vaccine. To better understand M2e immune-mediated protection, Fc receptor common γ chain deficient (FcR γ-/- ) and wild type mice were immunized with a tandem repeat of M2e presented on virus-like particles (M2e5x VLP). Levels of M2e-specific antibodies that were induced in FcR γ-/- mice after immunization with M2e5x VLP were similar to those in wild type mice. In addition, M2e antibodies induced in FcR γ-/- mice were found to be equally protective as compared to those induced in wild type mice. However, M2e5x VLP-immunized FcR γ-/- mice were not well protected as evidenced by severe weight loss, higher lung viral titers, and IL-6 inflammatory cytokine production upon influenza virus challenge infection compared to M2e5x VLP-immunized wild type mice. Importantly, FcR γ-/- mice that were immunized with inactivated influenza virus induced hemagglutination inhibition activity and were well protected without a significant weight loss. Interestingly, interferon-γ producing CD4 T and CD8 T cells were found to be high in lungs from M2e5x VLP immunized FcR γ-/- mice, which appeared to be correlated with a faster recovery after infection. These results indicate that Fc receptors play a primary role in conferring M2e specific antibody-mediated protection whereas T cells may contribute to the recovery at later stages of infection. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
KEYWORDS:
Fc receptor, Influenza virus, M2e VLPs, M2e antibodies, T cells
PMID:
24773389
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24773389