tetano
Editor, Senior Moderator
Vaccine. 2019 May 14. pii: S0264-410X(19)30632-2. doi: 10.1016/j.vaccine.2019.05.018. [Epub ahead of print]
[h=1]Flagellin-expressing virus-like particles exhibit adjuvant effects on promoting IgG isotype-switched long-lasting antibody induction and protection of influenza vaccines in CD4-deficient mice.[/h] Ko EJ[SUP]1[/SUP], Lee Y[SUP]2[/SUP], Lee YT[SUP]2[/SUP], Jung YJ[SUP]3[/SUP], Ngo VL[SUP]4[/SUP], Kim MC[SUP]5[/SUP], Kim KH[SUP]2[/SUP], Wang BZ[SUP]6[/SUP], Gewirtz AT[SUP]7[/SUP], Kang SM[SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Incorporation of membrane-anchored flagellin molecules into the surfaces of influenza virus-like particles (VLP) was previously reported to promote T helper (Th) 1-biased IgG antibody production and protective efficacy of co-presented vaccine antigens. Herein, we investigated the potential adjuvant effects and mechanisms of flagellin-expressing VLP (FliC-VLP) as an independent component on influenza vaccination in wild-type and mutant mouse models. FliC-VLP adjuvanted influenza vaccination was highly effective in promoting the induction of Th1-biased IgG isotype switched antibodies, enhanced protection, and long-lasting IgG antibody responses in both wild-type and CD4-knockout mice. In contrast, the adjuvant effects of soluble flagellin were Th2-biased and required CD4 T helper cells. The adjuvant effects of FliC-VLP were less dependent on CD4 T cells and flagellin-mediated innate immune signaling pathways. The results suggest that FliC-VLP might play an effective adjuvant role in an immune competent condition as well as in a defect of CD4 T cells.
Copyright ? 2019. Published by Elsevier Ltd.
[h=4]KEYWORDS:[/h] Adjuvant; CD4 T cells; Flagellin; Influenza; VLP
PMID: 31101421 DOI: 10.1016/j.vaccine.2019.05.018
[h=1]Flagellin-expressing virus-like particles exhibit adjuvant effects on promoting IgG isotype-switched long-lasting antibody induction and protection of influenza vaccines in CD4-deficient mice.[/h] Ko EJ[SUP]1[/SUP], Lee Y[SUP]2[/SUP], Lee YT[SUP]2[/SUP], Jung YJ[SUP]3[/SUP], Ngo VL[SUP]4[/SUP], Kim MC[SUP]5[/SUP], Kim KH[SUP]2[/SUP], Wang BZ[SUP]6[/SUP], Gewirtz AT[SUP]7[/SUP], Kang SM[SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Incorporation of membrane-anchored flagellin molecules into the surfaces of influenza virus-like particles (VLP) was previously reported to promote T helper (Th) 1-biased IgG antibody production and protective efficacy of co-presented vaccine antigens. Herein, we investigated the potential adjuvant effects and mechanisms of flagellin-expressing VLP (FliC-VLP) as an independent component on influenza vaccination in wild-type and mutant mouse models. FliC-VLP adjuvanted influenza vaccination was highly effective in promoting the induction of Th1-biased IgG isotype switched antibodies, enhanced protection, and long-lasting IgG antibody responses in both wild-type and CD4-knockout mice. In contrast, the adjuvant effects of soluble flagellin were Th2-biased and required CD4 T helper cells. The adjuvant effects of FliC-VLP were less dependent on CD4 T cells and flagellin-mediated innate immune signaling pathways. The results suggest that FliC-VLP might play an effective adjuvant role in an immune competent condition as well as in a defect of CD4 T cells.
Copyright ? 2019. Published by Elsevier Ltd.
[h=4]KEYWORDS:[/h] Adjuvant; CD4 T cells; Flagellin; Influenza; VLP
PMID: 31101421 DOI: 10.1016/j.vaccine.2019.05.018