tetano
Editor, Senior Moderator
Front Immunol
. 2022 Jan 11;12:815651.
doi: 10.3389/fimmu.2021.815651. eCollection 2021.
A Differential Signature of Circulating miRNAs and Cytokines Between COVID-19 and Community-Acquired Pneumonia Uncovers Novel Physiopathological Mechanisms of COVID-19
Pedro Martínez-Fleta[SUP] 1 [/SUP], Paula Vera-Tomé[SUP] 1 [/SUP], María Jiménez-Fernández[SUP] 1 [/SUP], Silvia Requena[SUP] 1 [/SUP], Emilia Roy-Vallejo[SUP] 2 [/SUP], Ancor Sanz-García[SUP] 3 [/SUP], Marta Lozano-Prieto[SUP] 1 [/SUP], Celia López-Sanz[SUP] 1 [/SUP], Alicia Vara[SUP] 1 [/SUP], Ángel Lancho-Sánchez[SUP] 4 [/SUP], Enrique Martín-Gayo[SUP] 1 5 [/SUP], Cecilia Muñoz-Calleja[SUP] 1 5 [/SUP], Arantzazu Alfranca[SUP] 1 6 [/SUP], Isidoro González-Álvaro[SUP] 1 7 [/SUP], José María Galván-Román[SUP] 2 [/SUP], Javier Aspa[SUP] 8 [/SUP], Hortensia de la Fuente[SUP] 1 6 [/SUP], Francisco Sánchez-Madrid[SUP] 1 5 6 [/SUP]
Affiliations
Abstract
Coronavirus Disease 2019 (COVID-19) pneumonia is a life-threatening infectious disease, especially for elderly patients with multiple comorbidities. Despite enormous efforts to understand its underlying etiopathogenic mechanisms, most of them remain elusive. In this study, we compared differential plasma miRNAs and cytokines profiles between COVID-19 and other community-acquired pneumonias (CAP). A first screening and subsequent validation assays in an independent cohort of patients revealed a signature of 15 dysregulated miRNAs between COVID-19 and CAP patients. Additionally, multivariate analysis displayed a combination of 4 miRNAs (miR-106b-5p, miR-221-3p, miR-25-3p and miR-30a-5p) that significantly discriminated between both pathologies. Search for targets of these miRNAs, combined with plasma protein measurements, identified a differential cytokine signature between COVID-19 and CAP that included EGFR, CXCL12 and IL-10. Significant differences were also detected in plasma levels of CXCL12, IL-17, TIMP-2 and IL-21R between mild and severe COVID-19 patients. These findings provide new insights into the etiopathological mechanisms underlying COVID-19.
Keywords: COVID-19; community-acquired pneumonia; microRNAs; plasma; soluble proteins.
. 2022 Jan 11;12:815651.
doi: 10.3389/fimmu.2021.815651. eCollection 2021.
A Differential Signature of Circulating miRNAs and Cytokines Between COVID-19 and Community-Acquired Pneumonia Uncovers Novel Physiopathological Mechanisms of COVID-19
Pedro Martínez-Fleta[SUP] 1 [/SUP], Paula Vera-Tomé[SUP] 1 [/SUP], María Jiménez-Fernández[SUP] 1 [/SUP], Silvia Requena[SUP] 1 [/SUP], Emilia Roy-Vallejo[SUP] 2 [/SUP], Ancor Sanz-García[SUP] 3 [/SUP], Marta Lozano-Prieto[SUP] 1 [/SUP], Celia López-Sanz[SUP] 1 [/SUP], Alicia Vara[SUP] 1 [/SUP], Ángel Lancho-Sánchez[SUP] 4 [/SUP], Enrique Martín-Gayo[SUP] 1 5 [/SUP], Cecilia Muñoz-Calleja[SUP] 1 5 [/SUP], Arantzazu Alfranca[SUP] 1 6 [/SUP], Isidoro González-Álvaro[SUP] 1 7 [/SUP], José María Galván-Román[SUP] 2 [/SUP], Javier Aspa[SUP] 8 [/SUP], Hortensia de la Fuente[SUP] 1 6 [/SUP], Francisco Sánchez-Madrid[SUP] 1 5 6 [/SUP]
Affiliations
- PMID: 35087533
- PMCID: PMC8787267
- DOI: 10.3389/fimmu.2021.815651
Abstract
Coronavirus Disease 2019 (COVID-19) pneumonia is a life-threatening infectious disease, especially for elderly patients with multiple comorbidities. Despite enormous efforts to understand its underlying etiopathogenic mechanisms, most of them remain elusive. In this study, we compared differential plasma miRNAs and cytokines profiles between COVID-19 and other community-acquired pneumonias (CAP). A first screening and subsequent validation assays in an independent cohort of patients revealed a signature of 15 dysregulated miRNAs between COVID-19 and CAP patients. Additionally, multivariate analysis displayed a combination of 4 miRNAs (miR-106b-5p, miR-221-3p, miR-25-3p and miR-30a-5p) that significantly discriminated between both pathologies. Search for targets of these miRNAs, combined with plasma protein measurements, identified a differential cytokine signature between COVID-19 and CAP that included EGFR, CXCL12 and IL-10. Significant differences were also detected in plasma levels of CXCL12, IL-17, TIMP-2 and IL-21R between mild and severe COVID-19 patients. These findings provide new insights into the etiopathological mechanisms underlying COVID-19.
Keywords: COVID-19; community-acquired pneumonia; microRNAs; plasma; soluble proteins.