tetano
Editor, Senior Moderator
Front Immunol
. 2022 Mar 29;13:841739.
doi: 10.3389/fimmu.2022.841739. eCollection 2022.
Alterations of Urinary Microbial Metabolites and Immune Indexes Linked With COVID-19 Infection and Prognosis
Yixian Jing[SUP] 1 [/SUP], Jing Wang[SUP] 2 [/SUP], Haiyan Zhang[SUP] 2 [/SUP], Kun Yang[SUP] 2 [/SUP], Jungang Li[SUP] 2 [/SUP], Ting Zhao[SUP] 1 [/SUP], Jiaxiu Liu[SUP] 2 [/SUP], Jing Wu[SUP] 3 4 [/SUP], Yaokai Chen[SUP] 1 [/SUP]
Affiliations
Abstract
Coronavirus disease 2019 (COVID-19) has evolved into an established global pandemic. Metabolomic studies in COVID-19 patients is worth exploring for further available screening methods. In our study, we recruited a study cohort of 350 subjects comprising 248 COVID-19 patients (161 non-severe cases, 60 asymptomatic cases, and 27 severe cases) and 102 healthy controls (HCs), and herein present data with respect to their demographic features, urinary metabolome, immunological indices, and follow-up health status. We found that COVID-19 resulted in alterations of 39 urinary, mainly microbial, metabolites. Using random forest analysis, a simplified marker panel including three microbial metabolites (oxoglutaric acid, indoxyl, and phenylacetamide) was constructed (AUC=0.963, 95% CI, 0.930-0.983), which exhibited higher diagnostic performance than immune feature-based panels between COVID-19 and HC groups (P<0.0001). Meanwhile, we observed that urine metabolic markers enabled discriminating asymptomatic patients (ASY) from HCs (AUC = 0.981, 95% CI, 0.946-0.996), and predicting the incidence of high-risk sequalae in COVID-19 individuals (AUC=0.931, 95% CI, 0.877-0.966). Co-expression network analysis showed that 13 urinary microbial metabolites (e.g., oxoglutaric acid) were significantly correlated with alterations of CD4[SUP]+[/SUP], CD3[SUP]+[/SUP], and CD8[SUP]+[/SUP] T-cells, as well as IFN-γ, IL-2 and IL-4 levels, suggesting close interactions between microbial metabolites and host immune dysregulation in COVID-19. Taken together, our findings indicate that urinary metabolites may have promising potential for screening of COVID-19 in different application scenarios, and provide a new entry point to understand the microbial metabolites and related immune dysfunction in COVID-19.
Keywords: COVID-19; diagnostic performance; high-risk sequelae; high-risk sequelae COVID-19; immune dysfunction; urinary microbial metabolites.
. 2022 Mar 29;13:841739.
doi: 10.3389/fimmu.2022.841739. eCollection 2022.
Alterations of Urinary Microbial Metabolites and Immune Indexes Linked With COVID-19 Infection and Prognosis
Yixian Jing[SUP] 1 [/SUP], Jing Wang[SUP] 2 [/SUP], Haiyan Zhang[SUP] 2 [/SUP], Kun Yang[SUP] 2 [/SUP], Jungang Li[SUP] 2 [/SUP], Ting Zhao[SUP] 1 [/SUP], Jiaxiu Liu[SUP] 2 [/SUP], Jing Wu[SUP] 3 4 [/SUP], Yaokai Chen[SUP] 1 [/SUP]
Affiliations
- PMID: 35422810
- PMCID: PMC9001849
- DOI: 10.3389/fimmu.2022.841739
Abstract
Coronavirus disease 2019 (COVID-19) has evolved into an established global pandemic. Metabolomic studies in COVID-19 patients is worth exploring for further available screening methods. In our study, we recruited a study cohort of 350 subjects comprising 248 COVID-19 patients (161 non-severe cases, 60 asymptomatic cases, and 27 severe cases) and 102 healthy controls (HCs), and herein present data with respect to their demographic features, urinary metabolome, immunological indices, and follow-up health status. We found that COVID-19 resulted in alterations of 39 urinary, mainly microbial, metabolites. Using random forest analysis, a simplified marker panel including three microbial metabolites (oxoglutaric acid, indoxyl, and phenylacetamide) was constructed (AUC=0.963, 95% CI, 0.930-0.983), which exhibited higher diagnostic performance than immune feature-based panels between COVID-19 and HC groups (P<0.0001). Meanwhile, we observed that urine metabolic markers enabled discriminating asymptomatic patients (ASY) from HCs (AUC = 0.981, 95% CI, 0.946-0.996), and predicting the incidence of high-risk sequalae in COVID-19 individuals (AUC=0.931, 95% CI, 0.877-0.966). Co-expression network analysis showed that 13 urinary microbial metabolites (e.g., oxoglutaric acid) were significantly correlated with alterations of CD4[SUP]+[/SUP], CD3[SUP]+[/SUP], and CD8[SUP]+[/SUP] T-cells, as well as IFN-γ, IL-2 and IL-4 levels, suggesting close interactions between microbial metabolites and host immune dysregulation in COVID-19. Taken together, our findings indicate that urinary metabolites may have promising potential for screening of COVID-19 in different application scenarios, and provide a new entry point to understand the microbial metabolites and related immune dysfunction in COVID-19.
Keywords: COVID-19; diagnostic performance; high-risk sequelae; high-risk sequelae COVID-19; immune dysfunction; urinary microbial metabolites.