tetano
Editor, Senior Moderator
Front Immunol
. 2023 Feb 23;14:1034978.
doi: 10.3389/fimmu.2023.1034978. eCollection 2023.
B cell receptor repertoire analysis from autopsy samples of COVID-19 patients
Sadahiro Iwabuchi[SUP] 1 [/SUP], Tomohide Tsukahara[SUP] 2 [/SUP], Toshitugu Okayama[SUP] 3 [/SUP], Masahiro Kitabatake[SUP] 4 [/SUP], Hideki Motobayashi[SUP] 5 [/SUP], Shigeyuki Shichino[SUP] 6 [/SUP], Tadashi Imafuku[SUP] 1 [/SUP], Kenzaburo Yamaji[SUP] 7 [/SUP], Kyohei Miyamoto[SUP] 8 [/SUP], Shinobu Tamura[SUP] 8 [/SUP], Satoshi Ueha[SUP] 6 [/SUP], Toshihiro Ito[SUP] 4 [/SUP], Shin-Ichi Murata[SUP] 9 [/SUP], Toshikazu Kondo[SUP] 10 [/SUP], Kazuho Ikeo[SUP] 3 [/SUP], Yutaka Suzuki[SUP] 11 [/SUP], Kouji Matsushima[SUP] 6 [/SUP], Michinori Kohara[SUP] 7 [/SUP], Toshihiko Torigoe[SUP] 2 [/SUP], Hiroki Yamaue[SUP] 5 12 [/SUP], Shinichi Hashimoto[SUP] 1 [/SUP]
Affiliations
Abstract
Neutralizing antibodies against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are being developed world over. We investigated the possibility of producing artificial antibodies from the formalin fixation and paraffin-embedding (FFPE) lung lobes of a patient who died by coronavirus disease 2019 (COVID-19). The B-cell receptors repertoire in the lung tissue where SARS-CoV-2 was detected were considered to have highly sensitive virus-neutralizing activity, and artificial antibodies were produced by combining the most frequently detected heavy and light chains. Some neutralizing effects against the SARS-CoV-2 were observed, and mixing two different artificial antibodies had a higher tendency to suppress the virus. The neutralizing effects were similar to the immunoglobulin G obtained from healthy donors who had received a COVID-19 mRNA vaccine. Therefore, the use of FFPE lung tissue, which preserves the condition of direct virus sensitization, to generate artificial antibodies may be useful against future unknown infectious diseases.
Keywords: B cell receptors; COVID-19; lung; serum IgGs; single cell; vaccine intake.
. 2023 Feb 23;14:1034978.
doi: 10.3389/fimmu.2023.1034978. eCollection 2023.
B cell receptor repertoire analysis from autopsy samples of COVID-19 patients
Sadahiro Iwabuchi[SUP] 1 [/SUP], Tomohide Tsukahara[SUP] 2 [/SUP], Toshitugu Okayama[SUP] 3 [/SUP], Masahiro Kitabatake[SUP] 4 [/SUP], Hideki Motobayashi[SUP] 5 [/SUP], Shigeyuki Shichino[SUP] 6 [/SUP], Tadashi Imafuku[SUP] 1 [/SUP], Kenzaburo Yamaji[SUP] 7 [/SUP], Kyohei Miyamoto[SUP] 8 [/SUP], Shinobu Tamura[SUP] 8 [/SUP], Satoshi Ueha[SUP] 6 [/SUP], Toshihiro Ito[SUP] 4 [/SUP], Shin-Ichi Murata[SUP] 9 [/SUP], Toshikazu Kondo[SUP] 10 [/SUP], Kazuho Ikeo[SUP] 3 [/SUP], Yutaka Suzuki[SUP] 11 [/SUP], Kouji Matsushima[SUP] 6 [/SUP], Michinori Kohara[SUP] 7 [/SUP], Toshihiko Torigoe[SUP] 2 [/SUP], Hiroki Yamaue[SUP] 5 12 [/SUP], Shinichi Hashimoto[SUP] 1 [/SUP]
Affiliations
- PMID: 36911681
- PMCID: PMC9996338
- DOI: 10.3389/fimmu.2023.1034978
Abstract
Neutralizing antibodies against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are being developed world over. We investigated the possibility of producing artificial antibodies from the formalin fixation and paraffin-embedding (FFPE) lung lobes of a patient who died by coronavirus disease 2019 (COVID-19). The B-cell receptors repertoire in the lung tissue where SARS-CoV-2 was detected were considered to have highly sensitive virus-neutralizing activity, and artificial antibodies were produced by combining the most frequently detected heavy and light chains. Some neutralizing effects against the SARS-CoV-2 were observed, and mixing two different artificial antibodies had a higher tendency to suppress the virus. The neutralizing effects were similar to the immunoglobulin G obtained from healthy donors who had received a COVID-19 mRNA vaccine. Therefore, the use of FFPE lung tissue, which preserves the condition of direct virus sensitization, to generate artificial antibodies may be useful against future unknown infectious diseases.
Keywords: B cell receptors; COVID-19; lung; serum IgGs; single cell; vaccine intake.