tetano
Editor, Senior Moderator
Front Immunol
. 2022 Feb 24;13:773652.
doi: 10.3389/fimmu.2022.773652. eCollection 2022.
Cross-Neutralizing Breadth and Longevity Against SARS-CoV-2 Variants After Infections
Yukiya Kurahashi[SUP] 1 [/SUP], Silvia Sutandhio[SUP] 1 [/SUP], Koichi Furukawa[SUP] 1 [/SUP], Lidya Handayani Tjan[SUP] 1 [/SUP], Sachiyo Iwata[SUP] 2 [/SUP], Shigeru Sano[SUP] 3 [/SUP], Yoshiki Tohma[SUP] 3 [/SUP], Hiroyuki Ohkita[SUP] 4 [/SUP], Sachiko Nakamura[SUP] 4 [/SUP], Mitsuhiro Nishimura[SUP] 1 [/SUP], Jun Arii[SUP] 1 [/SUP], Tatsunori Kiriu[SUP] 5 [/SUP], Masatsugu Yamamoto[SUP] 6 [/SUP], Tatsuya Nagano[SUP] 6 [/SUP], Yoshihiro Nishimura[SUP] 6 [/SUP], Yasuko Mori[SUP] 1 [/SUP]
Affiliations
Abstract
Background: Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the virus responsible for the Coronavirus Disease 2019 (COVID-19) pandemic. The emergence of variants of concern (VOCs) has become one of the most pressing issues in public health. To control VOCs, it is important to know which COVID-19 convalescent sera have cross-neutralizing activity against VOCs and how long the sera maintain this protective activity.
Methods: Sera of patients infected with SARS-CoV-2 from March 2020 to January 2021 and admitted to Hyogo Prefectural Kakogawa Medical Center were selected. Blood was drawn from patients at 1-3, 3-6, and 6-8 months post onset. Then, a virus neutralization assay against SARS-CoV-2 variants (D614G mutation as conventional strain; B.1.1.7, P.1, and B.1.351 as VOCs) was performed using authentic viruses.
Results: We assessed 97 sera from 42 patients. Sera from 28 patients showed neutralizing activity that was sustained for 3-8 months post onset. The neutralizing antibody titer against D614G significantly decreased in sera of 6-8 months post onset compared to those of 1-3 months post onset. However, the neutralizing antibody titers against the three VOCs were not significantly different among 1-3, 3-6, and 6-8 months post onset.
Discussion: Our results indicate that neutralizing antibodies that recognize the common epitope for several variants may be maintained for a long time, while neutralizing antibodies having specific epitopes for a variant, produced in large quantities immediately after infection, may decrease quite rapidly.
Keywords: COVID-19; SARS-CoV-2; breadth; neutralization; variants.
. 2022 Feb 24;13:773652.
doi: 10.3389/fimmu.2022.773652. eCollection 2022.
Cross-Neutralizing Breadth and Longevity Against SARS-CoV-2 Variants After Infections
Yukiya Kurahashi[SUP] 1 [/SUP], Silvia Sutandhio[SUP] 1 [/SUP], Koichi Furukawa[SUP] 1 [/SUP], Lidya Handayani Tjan[SUP] 1 [/SUP], Sachiyo Iwata[SUP] 2 [/SUP], Shigeru Sano[SUP] 3 [/SUP], Yoshiki Tohma[SUP] 3 [/SUP], Hiroyuki Ohkita[SUP] 4 [/SUP], Sachiko Nakamura[SUP] 4 [/SUP], Mitsuhiro Nishimura[SUP] 1 [/SUP], Jun Arii[SUP] 1 [/SUP], Tatsunori Kiriu[SUP] 5 [/SUP], Masatsugu Yamamoto[SUP] 6 [/SUP], Tatsuya Nagano[SUP] 6 [/SUP], Yoshihiro Nishimura[SUP] 6 [/SUP], Yasuko Mori[SUP] 1 [/SUP]
Affiliations
- PMID: 35281007
- PMCID: PMC8907139
- DOI: 10.3389/fimmu.2022.773652
Abstract
Background: Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the virus responsible for the Coronavirus Disease 2019 (COVID-19) pandemic. The emergence of variants of concern (VOCs) has become one of the most pressing issues in public health. To control VOCs, it is important to know which COVID-19 convalescent sera have cross-neutralizing activity against VOCs and how long the sera maintain this protective activity.
Methods: Sera of patients infected with SARS-CoV-2 from March 2020 to January 2021 and admitted to Hyogo Prefectural Kakogawa Medical Center were selected. Blood was drawn from patients at 1-3, 3-6, and 6-8 months post onset. Then, a virus neutralization assay against SARS-CoV-2 variants (D614G mutation as conventional strain; B.1.1.7, P.1, and B.1.351 as VOCs) was performed using authentic viruses.
Results: We assessed 97 sera from 42 patients. Sera from 28 patients showed neutralizing activity that was sustained for 3-8 months post onset. The neutralizing antibody titer against D614G significantly decreased in sera of 6-8 months post onset compared to those of 1-3 months post onset. However, the neutralizing antibody titers against the three VOCs were not significantly different among 1-3, 3-6, and 6-8 months post onset.
Discussion: Our results indicate that neutralizing antibodies that recognize the common epitope for several variants may be maintained for a long time, while neutralizing antibodies having specific epitopes for a variant, produced in large quantities immediately after infection, may decrease quite rapidly.
Keywords: COVID-19; SARS-CoV-2; breadth; neutralization; variants.