tetano
Editor, Senior Moderator
Front Immunol
. 2024 Sep 6:15:1413704.
doi: 10.3389/fimmu.2024.1413704. eCollection 2024. RNA editing in response to COVID-19 vaccines: unveiling dynamic epigenetic regulation of host immunity
Yun-Yun Jin[SUP] 1 2 3 [/SUP], Ya-Ping Liang[SUP] 1 2 3 [/SUP], Jia-Qi Pan[SUP] 1 2 3 [/SUP], Wen-Hao Huang[SUP] 1 2 3 [/SUP], Yan-Meng Feng[SUP] 1 2 3 [/SUP], Wei-Jia Sui[SUP] 1 2 3 [/SUP], Han Yu[SUP] 1 2 3 [/SUP], Xiao-Dan Tang[SUP] 1 2 3 [/SUP], Lin Zhu[SUP] 1 2 3 [/SUP], Jian-Huan Chen[SUP] 1 2 3 [/SUP]
Affiliations
Background: COVID-19 vaccines are crucial for reducing the threat and burden of the pandemic on global public health, yet the epigenetic, especially RNA editing in response to the vaccines remains unelucidated.
Results: Our current study performed an epitranscriptomic analysis of RNA-Seq data of 260 blood samples from 102 healthy and SARS-CoV-2 naïve individuals receiving different doses of the COVID-19 vaccine and revealed dynamic, transcriptome-wide adenosine to inosine (A-to-I) RNA editing changes in response to COVID-19 vaccines (RNA editing in response to COVID-19 vaccines). 5592 differential RNA editing (DRE) sites in 1820 genes were identified, with most of them showing up-regulated RNA editing and correlated with increased expression of edited genes. These deferentially edited genes were primarily involved in immune- and virus-related gene functions and pathways. Differential ADAR expression probably contributed to RNA editing in response to COVID-19 vaccines. One of the most significant DRE in RNA editing in response to COVID-19 vaccines was in apolipoprotein L6 (APOL6) 3' UTR, which positively correlated with its up-regulated expression. In addition, recoded key antiviral and immune-related proteins such as IFI30 and GBP1 recoded by missense editing was observed as an essential component of RNA editing in response to COVID-19 vaccines. Furthermore, both RNA editing in response to COVID-19 vaccines and its functions dynamically depended on the number of vaccine doses.
Conclusion: Our results thus underscored the potential impact of blood RNA editing in response to COVID-19 vaccines on the host's molecular immune system.
Keywords: A-to-I RNA editing; ADARs; COVID-19 vaccine; SARS-CoV-2; immune
. 2024 Sep 6:15:1413704.
doi: 10.3389/fimmu.2024.1413704. eCollection 2024. RNA editing in response to COVID-19 vaccines: unveiling dynamic epigenetic regulation of host immunity
Yun-Yun Jin[SUP] 1 2 3 [/SUP], Ya-Ping Liang[SUP] 1 2 3 [/SUP], Jia-Qi Pan[SUP] 1 2 3 [/SUP], Wen-Hao Huang[SUP] 1 2 3 [/SUP], Yan-Meng Feng[SUP] 1 2 3 [/SUP], Wei-Jia Sui[SUP] 1 2 3 [/SUP], Han Yu[SUP] 1 2 3 [/SUP], Xiao-Dan Tang[SUP] 1 2 3 [/SUP], Lin Zhu[SUP] 1 2 3 [/SUP], Jian-Huan Chen[SUP] 1 2 3 [/SUP]
Affiliations
- PMID: 39308856
- PMCID: PMC11413487
- DOI: 10.3389/fimmu.2024.1413704
Background: COVID-19 vaccines are crucial for reducing the threat and burden of the pandemic on global public health, yet the epigenetic, especially RNA editing in response to the vaccines remains unelucidated.
Results: Our current study performed an epitranscriptomic analysis of RNA-Seq data of 260 blood samples from 102 healthy and SARS-CoV-2 naïve individuals receiving different doses of the COVID-19 vaccine and revealed dynamic, transcriptome-wide adenosine to inosine (A-to-I) RNA editing changes in response to COVID-19 vaccines (RNA editing in response to COVID-19 vaccines). 5592 differential RNA editing (DRE) sites in 1820 genes were identified, with most of them showing up-regulated RNA editing and correlated with increased expression of edited genes. These deferentially edited genes were primarily involved in immune- and virus-related gene functions and pathways. Differential ADAR expression probably contributed to RNA editing in response to COVID-19 vaccines. One of the most significant DRE in RNA editing in response to COVID-19 vaccines was in apolipoprotein L6 (APOL6) 3' UTR, which positively correlated with its up-regulated expression. In addition, recoded key antiviral and immune-related proteins such as IFI30 and GBP1 recoded by missense editing was observed as an essential component of RNA editing in response to COVID-19 vaccines. Furthermore, both RNA editing in response to COVID-19 vaccines and its functions dynamically depended on the number of vaccine doses.
Conclusion: Our results thus underscored the potential impact of blood RNA editing in response to COVID-19 vaccines on the host's molecular immune system.
Keywords: A-to-I RNA editing; ADARs; COVID-19 vaccine; SARS-CoV-2; immune