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Front Immunol . Sex-biased immunogenicity of a mucosal subunit vaccine against SARS-CoV-2 in mice

tetano

Editor, Senior Moderator
Front Immunol


. 2024 May 21:15:1386243.
doi: 10.3389/fimmu.2024.1386243. eCollection 2024. Sex-biased immunogenicity of a mucosal subunit vaccine against SARS-CoV-2 in mice

Jianping Li[SUP] 1 [/SUP], Kevin S Hsu[SUP] 1 [/SUP], Savannah E Howe[SUP] 1 [/SUP], Tanya Hoang[SUP] 1 [/SUP], Zheng Xia[SUP] 1 [/SUP], Jay A Berzofsky[SUP] 1 [/SUP], Yongjun Sui[SUP] 1 [/SUP]



Affiliations
Abstract

Introduction: Current vaccines against COVID-19 administered via parenteral route have limited ability to induce mucosal immunity. There is a need for an effective mucosal vaccine to combat SARS-CoV-2 virus replication in the respiratory mucosa. Moreover, sex differences are known to affect systemic antibody responses against vaccines. However, their role in mucosal cellular responses against a vaccine remains unclear and is underappreciated.
Methods: We evaluated the mucosal immunogenicity of a booster vaccine regimen that is recombinant protein-based and administered intranasally in mice to explore sex differences in mucosal humoral and cellular responses.
Results: Our results showed that vaccinated mice elicited strong systemic antibody (Ab), nasal, and bronchiole alveolar lavage (BAL) IgA responses, and local T cell immune responses in the lung in a sex-biased manner irrespective of mouse genetic background. Monocytes, alveolar macrophages, and CD103+ resident dendritic cells (DCs) in the lungs are correlated with robust mucosal Ab and T cell responses induced by the mucosal vaccine.
Discussion: Our findings provide novel insights into optimizing next-generation booster vaccines against SARS-CoV-2 by inducing spike-specific lung T cell responses, as well as optimizing mucosal immunity for other respiratory infections, and a rationale for considering sex differences in future vaccine research and vaccination practice.

Keywords: SARS-CoV-2; and sex differences; innate immunity; lung tissue-resident T cells; mucosal vaccine.

 
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