tetano
Editor, Senior Moderator
Front Med (Lausanne)
. 2022 Feb 9;9:778489.
doi: 10.3389/fmed.2022.778489. eCollection 2022.
Long-Term Persisting SARS-CoV-2 RNA and Pathological Findings: Lessons Learnt From a Series of 35 COVID-19 Autopsies
Umberto Maccio[SUP] 1 [/SUP], Annelies S Zinkernagel[SUP] 2 [/SUP], Reto Schuepbach[SUP] 3 [/SUP], Elsbeth Probst-Mueller[SUP] 4 [/SUP], Karl Frontzek[SUP] 5 [/SUP], Silvio D Brugger[SUP] 2 [/SUP], Daniel Andrea Hofmaenner[SUP] 3 [/SUP], Holger Moch[SUP] 1 [/SUP], Zsuzsanna Varga[SUP] 1 [/SUP]
Affiliations
Abstract
Background: Long-term sequelae of coronavirus disease 2019 (COVID-19), including the interaction between persisting viral-RNA and specific tissue involvement, pose a challenging issue. In this study, we addressed the chronological correlation (after first clinical diagnosis and postmortem) between severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA and organ involvement.
Methods: The presence of postmortem SARS-CoV-2 RNA from 35 complete COVID-19 autopsies was correlated with the time interval between the first diagnosis of COVID-19 and death and with its relationship to morphologic findings.
Results: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA can be evident up to 40 days after the first diagnosis and can persist to 94 hours after death. Postmortem SARS-CoV-2 RNA was mostly positive in lungs (70%) and trachea (69%), but all investigated organs were positive with variable frequency. Late-stage tissue damage was evident up to 65 days after initial diagnosis in several organs. Positivity for SARS-CoV-2 RNA in pulmonary swabs correlated with diffuse alveolar damage (p = 0.0009). No correlation between positive swabs and other morphologic findings was present. Cerebral (p = 0.0003) and systemic hemorrhages (p = 0.009), cardiac thrombi (p = 0.04), and ischemic events (p = 0.03) were more frequent in the first wave, whereas bacterial pneumonia (p = 0.03) was more prevalent in the second wave. No differences in biometric data, clinical comorbidities, and other autopsy findings were found.
Conclusions: Our data provide evidence not only of long-term postmortem persisting SARS-CoV-2 RNA but also of tissue damage several weeks after the first diagnosis of SARS-CoV-2 infection. Additional conditions, such as concomitant bacterial pulmonary superinfection, lung aspergillosis, thromboembolic phenomena, and hemorrhages can further worsen tissue damage.
Keywords: COVID-19; SARS-CoV-2 RNA PCR; autopsy; histopathology; long-COVID; postmortal swabs; pulmonary superinfections.
. 2022 Feb 9;9:778489.
doi: 10.3389/fmed.2022.778489. eCollection 2022.
Long-Term Persisting SARS-CoV-2 RNA and Pathological Findings: Lessons Learnt From a Series of 35 COVID-19 Autopsies
Umberto Maccio[SUP] 1 [/SUP], Annelies S Zinkernagel[SUP] 2 [/SUP], Reto Schuepbach[SUP] 3 [/SUP], Elsbeth Probst-Mueller[SUP] 4 [/SUP], Karl Frontzek[SUP] 5 [/SUP], Silvio D Brugger[SUP] 2 [/SUP], Daniel Andrea Hofmaenner[SUP] 3 [/SUP], Holger Moch[SUP] 1 [/SUP], Zsuzsanna Varga[SUP] 1 [/SUP]
Affiliations
- PMID: 35223894
- PMCID: PMC8865372
- DOI: 10.3389/fmed.2022.778489
Abstract
Background: Long-term sequelae of coronavirus disease 2019 (COVID-19), including the interaction between persisting viral-RNA and specific tissue involvement, pose a challenging issue. In this study, we addressed the chronological correlation (after first clinical diagnosis and postmortem) between severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA and organ involvement.
Methods: The presence of postmortem SARS-CoV-2 RNA from 35 complete COVID-19 autopsies was correlated with the time interval between the first diagnosis of COVID-19 and death and with its relationship to morphologic findings.
Results: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA can be evident up to 40 days after the first diagnosis and can persist to 94 hours after death. Postmortem SARS-CoV-2 RNA was mostly positive in lungs (70%) and trachea (69%), but all investigated organs were positive with variable frequency. Late-stage tissue damage was evident up to 65 days after initial diagnosis in several organs. Positivity for SARS-CoV-2 RNA in pulmonary swabs correlated with diffuse alveolar damage (p = 0.0009). No correlation between positive swabs and other morphologic findings was present. Cerebral (p = 0.0003) and systemic hemorrhages (p = 0.009), cardiac thrombi (p = 0.04), and ischemic events (p = 0.03) were more frequent in the first wave, whereas bacterial pneumonia (p = 0.03) was more prevalent in the second wave. No differences in biometric data, clinical comorbidities, and other autopsy findings were found.
Conclusions: Our data provide evidence not only of long-term postmortem persisting SARS-CoV-2 RNA but also of tissue damage several weeks after the first diagnosis of SARS-CoV-2 infection. Additional conditions, such as concomitant bacterial pulmonary superinfection, lung aspergillosis, thromboembolic phenomena, and hemorrhages can further worsen tissue damage.
Keywords: COVID-19; SARS-CoV-2 RNA PCR; autopsy; histopathology; long-COVID; postmortal swabs; pulmonary superinfections.