tetano
Editor, Senior Moderator
Front Microbiol
. 2022 Apr 15;13:775186.
doi: 10.3389/fmicb.2022.775186. eCollection 2022.
Coronavirus Disease 2019-Related Alterations of Total and Anti-Spike IgG Glycosylation in Relation to Age and Anti-Spike IgG Titer
Christian Schwedler[SUP] 1 2 [/SUP], Marta Grzeski[SUP] 1 [/SUP], Kai Kappert[SUP] 1 3 [/SUP], Jörn Rust[SUP] 4 [/SUP], Guido Heymann[SUP] 5 [/SUP], Berthold Hoppe[SUP] 1 5 [/SUP], Véronique Blanchard[SUP] 1 [/SUP]
Affiliations
Abstract
The coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has been affecting the world since January 2020 and has caused millions of deaths. To gain a better insight into molecular changes underlying the COVID-19 disease, we investigated here the N-glycosylation of three immunoglobulin G (IgG) fractions isolated from plasma of 35 severe COVID-19 patients, namely total IgG[SUB]1[/SUB], total IgG[SUB]2[/SUB], and anti-Spike IgG, by means of MALDI-TOF-MS. All analyses were performed at the glycopeptide level to assure subclass- and site-specific information. For each COVID-19 patient, the analyses included three blood withdrawals at different time-points of hospitalization, which allowed profiling longitudinal alterations in IgG glycosylation. The COVID-19 patients presented altered IgG N-glycosylation profiles in all investigated IgG fractions. The most pronounced COVID-19-related changes were observed in the glycosylation profiles of antigen-specific anti-Spike IgG[SUB]1[/SUB]. Anti-Spike IgG[SUB]1[/SUB] fucosylation and galactosylation showed the strongest variation during the disease course, with the difference in anti-Spike IgG[SUB]1[/SUB] fucosylation being significantly correlated with patients' age. Decreases in anti-Spike IgG[SUB]1[/SUB] galactosylation and sialylation in the course of the disease were found to be significantly correlated with the difference in anti-Spike IgG plasma concentration. The present findings suggest that patients' age and anti-S IgG abundance might influence IgG N-glycosylation alterations occurring in COVID-19.
Keywords: COVID-19; IgG glycosylation; MALDI-TOF; N-glycopeptides; SARS-CoV-2; Spike; glycans.
. 2022 Apr 15;13:775186.
doi: 10.3389/fmicb.2022.775186. eCollection 2022.
Coronavirus Disease 2019-Related Alterations of Total and Anti-Spike IgG Glycosylation in Relation to Age and Anti-Spike IgG Titer
Christian Schwedler[SUP] 1 2 [/SUP], Marta Grzeski[SUP] 1 [/SUP], Kai Kappert[SUP] 1 3 [/SUP], Jörn Rust[SUP] 4 [/SUP], Guido Heymann[SUP] 5 [/SUP], Berthold Hoppe[SUP] 1 5 [/SUP], Véronique Blanchard[SUP] 1 [/SUP]
Affiliations
- PMID: 35495660
- PMCID: PMC9051488
- DOI: 10.3389/fmicb.2022.775186
Abstract
The coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has been affecting the world since January 2020 and has caused millions of deaths. To gain a better insight into molecular changes underlying the COVID-19 disease, we investigated here the N-glycosylation of three immunoglobulin G (IgG) fractions isolated from plasma of 35 severe COVID-19 patients, namely total IgG[SUB]1[/SUB], total IgG[SUB]2[/SUB], and anti-Spike IgG, by means of MALDI-TOF-MS. All analyses were performed at the glycopeptide level to assure subclass- and site-specific information. For each COVID-19 patient, the analyses included three blood withdrawals at different time-points of hospitalization, which allowed profiling longitudinal alterations in IgG glycosylation. The COVID-19 patients presented altered IgG N-glycosylation profiles in all investigated IgG fractions. The most pronounced COVID-19-related changes were observed in the glycosylation profiles of antigen-specific anti-Spike IgG[SUB]1[/SUB]. Anti-Spike IgG[SUB]1[/SUB] fucosylation and galactosylation showed the strongest variation during the disease course, with the difference in anti-Spike IgG[SUB]1[/SUB] fucosylation being significantly correlated with patients' age. Decreases in anti-Spike IgG[SUB]1[/SUB] galactosylation and sialylation in the course of the disease were found to be significantly correlated with the difference in anti-Spike IgG plasma concentration. The present findings suggest that patients' age and anti-S IgG abundance might influence IgG N-glycosylation alterations occurring in COVID-19.
Keywords: COVID-19; IgG glycosylation; MALDI-TOF; N-glycopeptides; SARS-CoV-2; Spike; glycans.