tetano
Editor, Senior Moderator
Future Virol
. 2020 Oct;10.2217/fvl-2020-0299.
doi: 10.2217/fvl-2020-0299.
A multiple-center clinical evaluation of a new real-time reverse transcriptase PCR diagnostic kit for SARS-CoV-2
Jing-Jing Guo[SUP] 1 [/SUP], Yan-Hua Yu[SUP] 2 [/SUP], Xue-Ying Ma[SUP] 1 [/SUP], Ya-Nan Liu[SUP] 1 [/SUP], Qian Fang[SUP] 1 [/SUP], Pei Qu[SUP] 1 [/SUP], Jie Guo[SUP] 1 [/SUP], Jin-Li Lou[SUP] 2 [/SUP], Ya-Jie Wang[SUP] 1 [/SUP]
Affiliations
Abstract
Aim: The outbreak of severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) has had serious repercussions worldwide. This study was aimed to evaluate the accuracy of a new kit for detection of SARS-CoV-2 compared with similar detection kit. Materials & methods: A total of 500 subjects were included and tested with both the new test and control kits. Clinical diagnosis results were taken as the reference standard. Results: Compared with clinical diagnosis, the sensitivity of the test kit was 82.64%, specificity was 98.45% and total coincidence rate was 90.80%. The total coincidence rate, sensitivity and specificity between control kit and clinical diagnosis were 89.20%, 78.10% and 99.61%, respectively. Conclusions: The new kit was comparable to the similar detection kit for detection of SARS-CoV-2 in sensitivity, specificity and total coincidence rate.
Keywords: SARS-CoV-2; detection; evaluation; real-time PCR Kit.
. 2020 Oct;10.2217/fvl-2020-0299.
doi: 10.2217/fvl-2020-0299.
A multiple-center clinical evaluation of a new real-time reverse transcriptase PCR diagnostic kit for SARS-CoV-2
Jing-Jing Guo[SUP] 1 [/SUP], Yan-Hua Yu[SUP] 2 [/SUP], Xue-Ying Ma[SUP] 1 [/SUP], Ya-Nan Liu[SUP] 1 [/SUP], Qian Fang[SUP] 1 [/SUP], Pei Qu[SUP] 1 [/SUP], Jie Guo[SUP] 1 [/SUP], Jin-Li Lou[SUP] 2 [/SUP], Ya-Jie Wang[SUP] 1 [/SUP]
Affiliations
- PMID: 33576582
- PMCID: PMC7594197
- DOI: 10.2217/fvl-2020-0299
Abstract
Aim: The outbreak of severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) has had serious repercussions worldwide. This study was aimed to evaluate the accuracy of a new kit for detection of SARS-CoV-2 compared with similar detection kit. Materials & methods: A total of 500 subjects were included and tested with both the new test and control kits. Clinical diagnosis results were taken as the reference standard. Results: Compared with clinical diagnosis, the sensitivity of the test kit was 82.64%, specificity was 98.45% and total coincidence rate was 90.80%. The total coincidence rate, sensitivity and specificity between control kit and clinical diagnosis were 89.20%, 78.10% and 99.61%, respectively. Conclusions: The new kit was comparable to the similar detection kit for detection of SARS-CoV-2 in sensitivity, specificity and total coincidence rate.
Keywords: SARS-CoV-2; detection; evaluation; real-time PCR Kit.