tetano
Editor, Senior Moderator
Future Virol
. 2020 Aug;15(8):515-524.
doi: 10.2217/fvl-2020-0169.
Investigation of compatibility of severe acute respiratory syndrome coronavirus 2 reverse transcriptase-PCR kits containing different gene targets during coronavirus disease 2019 pandemic
Figen Sarıg?l[SUP] 1 [/SUP], Osman Doluca[SUP] 2 [/SUP], Sıla Akhan[SUP] 3 [/SUP], Murat Sayan[SUP] 4 5 [/SUP]
Affiliations
Abstract
Aim: In the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), reverse transcriptase-PCR (RT-PCR) technique is often used. We evaluated the compatibility of SARS-CoV-2 RT-PCR kits containing different gene targets during the pandemic.
Materials & methods: Samples were tested by Bio-Speddy[SUP]?[/SUP] (RdRp gene) and Diagnovital[SUP]?[/SUP] (RdRp + E genes). The correlation between two assays were determined by Deming regression analysis and chi-square analyses.
Results: Diagnovital PCR kit showed amplification in a narrow Ct range and conveniently sharper exponential amplification curves than Bio-Speedy PCR kit. While the correlation between the findings of the two kits was apparent even with single gene target, this correlation increased when a secondary biomarker was added to the correlation calculations.
Conclusion: We have observed high correlation between different PCR kits, however, using different PCR kits during the pandemic may provide a more accurate diagnosis of SARS-CoV-2, since despite correlation there are a number of patients showing contradicting diagnosis.
Keywords: COVID-19; RT-PCR; SARS-CoV-2; coronavirus.
. 2020 Aug;15(8):515-524.
doi: 10.2217/fvl-2020-0169.
Investigation of compatibility of severe acute respiratory syndrome coronavirus 2 reverse transcriptase-PCR kits containing different gene targets during coronavirus disease 2019 pandemic
Figen Sarıg?l[SUP] 1 [/SUP], Osman Doluca[SUP] 2 [/SUP], Sıla Akhan[SUP] 3 [/SUP], Murat Sayan[SUP] 4 5 [/SUP]
Affiliations
- PMID: 33005213
- PMCID: PMC7507840
- DOI: 10.2217/fvl-2020-0169
Abstract
Aim: In the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), reverse transcriptase-PCR (RT-PCR) technique is often used. We evaluated the compatibility of SARS-CoV-2 RT-PCR kits containing different gene targets during the pandemic.
Materials & methods: Samples were tested by Bio-Speddy[SUP]?[/SUP] (RdRp gene) and Diagnovital[SUP]?[/SUP] (RdRp + E genes). The correlation between two assays were determined by Deming regression analysis and chi-square analyses.
Results: Diagnovital PCR kit showed amplification in a narrow Ct range and conveniently sharper exponential amplification curves than Bio-Speedy PCR kit. While the correlation between the findings of the two kits was apparent even with single gene target, this correlation increased when a secondary biomarker was added to the correlation calculations.
Conclusion: We have observed high correlation between different PCR kits, however, using different PCR kits during the pandemic may provide a more accurate diagnosis of SARS-CoV-2, since despite correlation there are a number of patients showing contradicting diagnosis.
Keywords: COVID-19; RT-PCR; SARS-CoV-2; coronavirus.