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Genetics, Receptor Binding Property, and Transmissibility in Mammals of Naturally Isolated H9N2 Avian Influenza Viruses

tetano

Editor, Senior Moderator
PLoS Pathog. 2014 Nov 20;10(11):e1004508. doi: 10.1371/journal.ppat.1004508. eCollection 2014.
Genetics, Receptor Binding Property, and Transmissibility in Mammals of Naturally Isolated H9N2 Avian Influenza Viruses.
Li X1, Shi J2, Guo J1, Deng G2, Zhang Q2, Wang J2, He X2, Wang K3, Chen J3, Li Y2, Fan J2, Kong H2, Gu C2, Guan Y2, Suzuki Y4, Kawaoka Y5, Liu L2, Jiang Y2, Tian G2, Li Y2, Bu Z2, Chen H2.
Author information
Abstract

H9N2 subtype influenza viruses have been detected in different species of wild birds and domestic poultry in many countries for several decades. Because these viruses are of low pathogenicity in poultry, their eradication is not a priority for animal disease control in many countries, which has allowed them to continue to evolve and spread. Here, we characterized the genetic variation, receptor-binding specificity, replication capability, and transmission in mammals of a series of H9N2 influenza viruses that were detected in live poultry markets in southern China between 2009 and 2013. Thirty-five viruses represented 17 genotypes on the basis of genomic diversity, and one specific "internal-gene-combination" predominated among the H9N2 viruses. This gene combination was also present in the H7N9 and H10N8 viruses that have infected humans in China. All of the 35 viruses preferentially bound to the human-like receptor, although two also retained the ability to bind to the avian-like receptor. Six of nine viruses tested were transmissible in ferrets by respiratory droplet; two were highly transmissible. Some H9N2 viruses readily acquired the 627K or 701N mutation in their PB2 gene upon infection of ferrets, further enhancing their virulence and transmission in mammals. Our study indicates that the widespread dissemination of H9N2 viruses poses a threat to human health not only because of the potential of these viruses to cause an influenza pandemic, but also because they can function as "vehicles" to deliver different subtypes of influenza viruses from avian species to humans.

PMID:
25411973
[PubMed - in process]

http://www.ncbi.nlm.nih.gov/pubmed/25411973
 
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