tetano
Editor, Senior Moderator
Science. 2018 Jan 19;359(6373):290-296. doi: 10.1126/science.aan8806.
[h=1]Genome-wide identification of interferon-sensitive mutations enables influenza vaccine design.[/h] Du Y[SUP]1,[/SUP][SUP]2[/SUP], Xin L[SUP]3[/SUP], Shi Y[SUP]4[/SUP], Zhang TH[SUP]4,[/SUP][SUP]5[/SUP], Wu NC[SUP]5[/SUP], Dai L[SUP]4[/SUP], Gong D[SUP]4[/SUP], Brar G[SUP]4[/SUP], Shu S[SUP]4[/SUP], Luo J[SUP]4,[/SUP][SUP]6,[/SUP][SUP]7[/SUP], Reiley W[SUP]8[/SUP], Tseng YW[SUP]4[/SUP], Bai H[SUP]3[/SUP], Wu TT[SUP]4[/SUP], Wang J[SUP]4,[/SUP][SUP]6[/SUP], Shu Y[SUP]3,[/SUP][SUP]9[/SUP], Sun R[SUP]1,[/SUP][SUP]2,[/SUP][SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] In conventional attenuated viral vaccines, immunogenicity is often suboptimal. Here we present a systematic approach for vaccine development that eliminates interferon (IFN)-modulating functions genome-wide while maintaining virus replication fitness. We applied a quantitative high-throughput genomics system to influenza A virus that simultaneously measured the replication fitness and IFN sensitivity of mutations across the entire genome. By incorporating eight IFN-sensitive mutations, we generated a hyper-interferon-sensitive (HIS) virus as a vaccine candidate. HIS virus is highly attenuated in IFN-competent hosts but able to induce transient IFN responses, elicits robust humoral and cellular immune responses, and provides protection against homologous and heterologous viral challenges. Our approach, which attenuates the virus and promotes immune responses concurrently, is broadly applicable for vaccine development against other pathogens.
PMID: 29348231 DOI: 10.1126/science.aan8806
[h=1]Genome-wide identification of interferon-sensitive mutations enables influenza vaccine design.[/h] Du Y[SUP]1,[/SUP][SUP]2[/SUP], Xin L[SUP]3[/SUP], Shi Y[SUP]4[/SUP], Zhang TH[SUP]4,[/SUP][SUP]5[/SUP], Wu NC[SUP]5[/SUP], Dai L[SUP]4[/SUP], Gong D[SUP]4[/SUP], Brar G[SUP]4[/SUP], Shu S[SUP]4[/SUP], Luo J[SUP]4,[/SUP][SUP]6,[/SUP][SUP]7[/SUP], Reiley W[SUP]8[/SUP], Tseng YW[SUP]4[/SUP], Bai H[SUP]3[/SUP], Wu TT[SUP]4[/SUP], Wang J[SUP]4,[/SUP][SUP]6[/SUP], Shu Y[SUP]3,[/SUP][SUP]9[/SUP], Sun R[SUP]1,[/SUP][SUP]2,[/SUP][SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] In conventional attenuated viral vaccines, immunogenicity is often suboptimal. Here we present a systematic approach for vaccine development that eliminates interferon (IFN)-modulating functions genome-wide while maintaining virus replication fitness. We applied a quantitative high-throughput genomics system to influenza A virus that simultaneously measured the replication fitness and IFN sensitivity of mutations across the entire genome. By incorporating eight IFN-sensitive mutations, we generated a hyper-interferon-sensitive (HIS) virus as a vaccine candidate. HIS virus is highly attenuated in IFN-competent hosts but able to induce transient IFN responses, elicits robust humoral and cellular immune responses, and provides protection against homologous and heterologous viral challenges. Our approach, which attenuates the virus and promotes immune responses concurrently, is broadly applicable for vaccine development against other pathogens.
PMID: 29348231 DOI: 10.1126/science.aan8806