Glyconanoparticles for the plasmonic detection and discrimination between human and avian influenza virus

[tetano]

Org Biomol Chem. 2013 Sep 3. [Epub ahead of print]
Glyconanoparticles for the plasmonic detection and discrimination between human and avian influenza virus.
Mar?n MJ, Rashid A, Rejzek M, Fairhurst SA, Wharton SA, Martin SR, McCauley JW, Wileman T, Field RA, Russell DA.
Source

School of Chemistry, University of East Anglia, Norwich Research Park, Norwich, Norfolk NR4 7TJ, UK. d.russell@uea.ac.uk.
Abstract

A plasmonic bioassay for the specific detection of human influenza virus has been developed based on gold nanoparticles functionalised with a designed and synthesised thiolated trivalent α2,6-thio-linked sialic acid derivative. The glyconanoparticles consist of the thiolated trivalent α2,6-thio-linked sialic acid derivative and a thiolated polyethylene glycol (PEG) derivative self-assembled onto the gold surface. Varying ratios of the trivalent α2,6-thio-linked sialic acid ligand and the PEG ligand were used; a ratio of 25 : 75 was found to be optimum for the detection of human influenza virus X31 (H3N2). In the presence of the influenza virus a solution of the glyconanoparticles aggregate following the binding of the trivalent α2,6-thio-linked sialic acid ligand to the haemagglutinin on the surface of the virus. The aggregation of the glycoparticles with the influenza virus induces a colour change of the solution within 30 min. Non-purified influenza virus in allantoic fluid was successfully detected using the functionalised glyconanoparticles. A comparison between the trivalent and a monovalent α2,6-thio-linked sialic acid functionalised nanoparticles confirmed that more rapid results, with greater sensitivity, were achieved using the trivalent ligand for the detection of the X31 virus. Importantly, the glyconanoparticles were able to discriminate between human (α2,6 binding) and avian (α2,3 binding) RG14 (H5N1) influenza virus highlighting the binding specificity of the trivalent α2,6-thio-linked sialic acid ligand.

PMID:
24057694
[PubMed - as supplied by publisher]

http://www.ncbi.nlm.nih.gov/pubmed/24057694

 

[Emily]

Re: Glyconanoparticles for the plasmonic detection and discrimination between human and avian influenza virus

http://www.medicaldaily.com/hunting-flu-gold-and-sugar-new-sensor-spots-influenza-within-half-hour-264583

Hunting The Flu With Gold And Sugar: New Sensor Spots Influenza Within Half An Hour
By John Ericson | Dec 8, 2013 05:59 PM EDT

The flu virus could soon become much easier to identify, according to a new study. Researchers at the University of East Anglia in the UK have discovered that a mix of sugar gold nanoparticles can detect influenza pathogens and differentiate between individual strains within 30 minutes. Aside from facilitating disease control, the innovative method could inspire a new generation of virus detecting devices...