tetano
Editor, Senior Moderator
Nanomedicine. 2018 Apr 9. pii: S1549-9634(18)30069-8. doi: 10.1016/j.nano.2018.03.007. [Epub ahead of print]
[h=1]Gold nanoparticles conjugating recombinant influenza hemagglutinin trimers and flagellin enhanced mucosal cellular immunity.[/h] Wang C[SUP]1[/SUP], Zhu W[SUP]1[/SUP], Luo Y[SUP]1[/SUP], Wang BZ[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The immunogenicity of subunit vaccines can be augmented by formulating them into nanoparticles. We conjugated recombinant trimetric influenza A/Aichi/2/68(H3N2) hemagglutinin (HA) onto functionalized gold nanoparticles (AuNPs) surfaces in a repetitive, oriented configuration. To further improve the immunogenicity, we generated Toll-like receptor 5 (TLR5) agonist flagellin (FliC)-coupled AuNPs as particulate adjuvants. Intranasal immunizations with an AuNP-HA and AuNP-FliC particle mixture elicited strong mucosal and systemic immune responses that protected hosts against lethal influenza challenges. Compared with the AuNP-HA alone group, the addition of AuNP-FliC improved mucosal B cell responses as characterized by elevated influenza specific IgA and IgG levels in nasal, tracheal, and lung washes. AuNP-HA/AuNP-FliC also stimulated antigen-specific interferon-γ (IFN-γ)-secreting CD4[SUP]+[/SUP] cell proliferation and induced strong effector CD8[SUP]+[/SUP] T cell activation. Our results indicate that intranasal co-delivery of antigen and adjuvant-displaying AuNPs enhanced vaccine efficacy by inducing potent cellular immune responses.
[h=4]KEYWORDS:[/h] adjuvant; cellular immunity; co-delivery; gold nanoparticle; mucosal vaccine
PMID: 29649593 DOI: 10.1016/j.nano.2018.03.007
[h=1]Gold nanoparticles conjugating recombinant influenza hemagglutinin trimers and flagellin enhanced mucosal cellular immunity.[/h] Wang C[SUP]1[/SUP], Zhu W[SUP]1[/SUP], Luo Y[SUP]1[/SUP], Wang BZ[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The immunogenicity of subunit vaccines can be augmented by formulating them into nanoparticles. We conjugated recombinant trimetric influenza A/Aichi/2/68(H3N2) hemagglutinin (HA) onto functionalized gold nanoparticles (AuNPs) surfaces in a repetitive, oriented configuration. To further improve the immunogenicity, we generated Toll-like receptor 5 (TLR5) agonist flagellin (FliC)-coupled AuNPs as particulate adjuvants. Intranasal immunizations with an AuNP-HA and AuNP-FliC particle mixture elicited strong mucosal and systemic immune responses that protected hosts against lethal influenza challenges. Compared with the AuNP-HA alone group, the addition of AuNP-FliC improved mucosal B cell responses as characterized by elevated influenza specific IgA and IgG levels in nasal, tracheal, and lung washes. AuNP-HA/AuNP-FliC also stimulated antigen-specific interferon-γ (IFN-γ)-secreting CD4[SUP]+[/SUP] cell proliferation and induced strong effector CD8[SUP]+[/SUP] T cell activation. Our results indicate that intranasal co-delivery of antigen and adjuvant-displaying AuNPs enhanced vaccine efficacy by inducing potent cellular immune responses.
[h=4]KEYWORDS:[/h] adjuvant; cellular immunity; co-delivery; gold nanoparticle; mucosal vaccine
PMID: 29649593 DOI: 10.1016/j.nano.2018.03.007