tetano
Editor, Senior Moderator
AIDS Res Ther. 2017 Feb 13;14(1):7. doi: 10.1186/s12981-017-0135-1.
[h=1]H1N1 influenza vaccination in HIV-infected women on effective antiretroviral treatment did not induce measurable antigen-driven proliferation of the HIV-1 proviral reservoir.[/h] Wagner TA[SUP]1,[/SUP][SUP]2[/SUP], Huang HC[SUP]3[/SUP], Salyer CE[SUP]4[/SUP], Richardson KM[SUP]5[/SUP], Weinberg A[SUP]5[/SUP], Nachman S[SUP]6[/SUP], Frenkel LM[SUP]7,[/SUP][SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]OBJECTIVES:[/h] Antigen-induced activation and proliferation of HIV-1-infected cells is hypothesized to be a mechanism of HIV persistence during antiretroviral therapy. The objective of this study was to determine if proliferation of H1N1-specific HIV-infected cells could be detected following H1N1 vaccination.
[h=4]METHODS:[/h] This study utilized cryopreserved PBMC from a previously conducted trial of H1N1 vaccination in HIV-infected pregnant women. HIV-1 DNA concentrations and 437 HIV-1 C2V5 env DNA sequences were analyzed from ten pregnant women on effective antiretroviral therapy, before and 21 days after H1N1 influenza vaccination.
[h=4]RESULTS:[/h] HIV-1 DNA concentration did not change after vaccination (median pre- vs. post-vaccination: 95.77 vs. 41.28 copies/million PBMC, p = .37). Analyses of sequences did not detect evidence of HIV replication or proliferation of infected cells.
[h=4]CONCLUSIONS:[/h] Antigenic stimulation during effective ART did not have a detectable effect on the genetic makeup of the HIV-1 DNA reservoir. Longitudinal comparison of the amount and integration sites of HIV-1 in antigen-specific cells to chronic infections (such as herpesviruses) may be needed to definitively evaluate whether antigenic stimulation induces proliferation of HIV-1 infected cells.
[h=4]KEYWORDS:[/h] Antiretroviral therapy; Antiretroviral treatment; H1N1; HIV; HIV DNA; Influenza; Latency; Pregnancy; Vaccination
PMID: 28193244 DOI: 10.1186/s12981-017-0135-1
[PubMed - in process]
[h=1]H1N1 influenza vaccination in HIV-infected women on effective antiretroviral treatment did not induce measurable antigen-driven proliferation of the HIV-1 proviral reservoir.[/h] Wagner TA[SUP]1,[/SUP][SUP]2[/SUP], Huang HC[SUP]3[/SUP], Salyer CE[SUP]4[/SUP], Richardson KM[SUP]5[/SUP], Weinberg A[SUP]5[/SUP], Nachman S[SUP]6[/SUP], Frenkel LM[SUP]7,[/SUP][SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]OBJECTIVES:[/h] Antigen-induced activation and proliferation of HIV-1-infected cells is hypothesized to be a mechanism of HIV persistence during antiretroviral therapy. The objective of this study was to determine if proliferation of H1N1-specific HIV-infected cells could be detected following H1N1 vaccination.
[h=4]METHODS:[/h] This study utilized cryopreserved PBMC from a previously conducted trial of H1N1 vaccination in HIV-infected pregnant women. HIV-1 DNA concentrations and 437 HIV-1 C2V5 env DNA sequences were analyzed from ten pregnant women on effective antiretroviral therapy, before and 21 days after H1N1 influenza vaccination.
[h=4]RESULTS:[/h] HIV-1 DNA concentration did not change after vaccination (median pre- vs. post-vaccination: 95.77 vs. 41.28 copies/million PBMC, p = .37). Analyses of sequences did not detect evidence of HIV replication or proliferation of infected cells.
[h=4]CONCLUSIONS:[/h] Antigenic stimulation during effective ART did not have a detectable effect on the genetic makeup of the HIV-1 DNA reservoir. Longitudinal comparison of the amount and integration sites of HIV-1 in antigen-specific cells to chronic infections (such as herpesviruses) may be needed to definitively evaluate whether antigenic stimulation induces proliferation of HIV-1 infected cells.
[h=4]KEYWORDS:[/h] Antiretroviral therapy; Antiretroviral treatment; H1N1; HIV; HIV DNA; Influenza; Latency; Pregnancy; Vaccination
PMID: 28193244 DOI: 10.1186/s12981-017-0135-1
[PubMed - in process]