tetano
Editor, Senior Moderator
Biomed Environ Sci. 2014 Oct;27(10):763-9. doi: 10.3967/bes2014.111.
H5N1 Avian Influenza Pre-pandemic Vaccine Strains in China.
Bo H, Dong LB, Zhang Y, Dong J, Zou SM, Gao RB, Wang da Y, Shu YL.
Author information
Abstract
OBJECTIVE:
To prepare the 4 candidate vaccine strains of H5N1 avian influenza virus isolated in China.
METHODS:
Recombinant viruses were rescued using reverse genetics. Neuraminidase (NA) and hemagglutinin (HA) segments of the A/Xinjiang/1/2006, A/Guangxi/1/2009, A/Hubei/1/2010, and A/Guangdong/1/2011 viruses were amplified by RT-PCR. Multibasic amino acid cleavage site of HA was removed and ligated into the pCIpolI vector for virus rescue. The recombinant viruses were evaluated by trypsin dependent assays. Their embryonate survival and antigenicity were compared with those of the respective wild-type viruses.
RESULTS:
The 4 recombinant viruses showed similar antigenicity compared with wild-type viruses, chicken embryo survival and trypsin-dependent characteristics.
CONCLUSION:
The 4 recombinant viruses rescued using reverse genetics meet the criteria for classification of low pathogenic avian influenza strains, thus supporting the use of them for the development of seeds and production of pre-pandemic vaccines.
Copyright ? 2014 The Editorial Board of Biomedical and Environmental Sciences. Published by China CDC. All rights reserved.
KEYWORDS:
H5N1; Influenza; Pre-pandemic; Vaccine strains
PMID:
25341811
[PubMed - in process]
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/25341811
H5N1 Avian Influenza Pre-pandemic Vaccine Strains in China.
Bo H, Dong LB, Zhang Y, Dong J, Zou SM, Gao RB, Wang da Y, Shu YL.
Author information
Abstract
OBJECTIVE:
To prepare the 4 candidate vaccine strains of H5N1 avian influenza virus isolated in China.
METHODS:
Recombinant viruses were rescued using reverse genetics. Neuraminidase (NA) and hemagglutinin (HA) segments of the A/Xinjiang/1/2006, A/Guangxi/1/2009, A/Hubei/1/2010, and A/Guangdong/1/2011 viruses were amplified by RT-PCR. Multibasic amino acid cleavage site of HA was removed and ligated into the pCIpolI vector for virus rescue. The recombinant viruses were evaluated by trypsin dependent assays. Their embryonate survival and antigenicity were compared with those of the respective wild-type viruses.
RESULTS:
The 4 recombinant viruses showed similar antigenicity compared with wild-type viruses, chicken embryo survival and trypsin-dependent characteristics.
CONCLUSION:
The 4 recombinant viruses rescued using reverse genetics meet the criteria for classification of low pathogenic avian influenza strains, thus supporting the use of them for the development of seeds and production of pre-pandemic vaccines.
Copyright ? 2014 The Editorial Board of Biomedical and Environmental Sciences. Published by China CDC. All rights reserved.
KEYWORDS:
H5N1; Influenza; Pre-pandemic; Vaccine strains
PMID:
25341811
[PubMed - in process]
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/25341811