tetano
Editor, Senior Moderator
Clin Vaccine Immunol. 2015 Feb 11. pii: CVI.00778-14. [Epub ahead of print]
[h=1]H9N2 Influenza Whole Inactivated Virus Combined with Polyethyleneimine Strongly Enhance Mucosal and Systemic Immunity after Intranasal Immunization in Mice.[/h] Qin T[SUP]1[/SUP], Yin Y[SUP]1[/SUP], Huang L[SUP]1[/SUP], Yu Q[SUP]1[/SUP], Yang Q[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza whole inactivated virus (WIV) are more immunogenic and induce protective antibody responses than other formulations like split virus (SV) or subunit (SU) vaccines, after intranasal mucosal delivery. Polyethyleneimine (PEI), an organic polycation, is widely used as a gene transfection and DNA vaccine delivery reagent. Although PEI has potent mucosal adjuvant activity for viral subunit glycoprotein antigens recently, its immune activity with H9N2 WIV is not well demonstrated. Here, mice were immunized intranasally with H9N2 WIV combined with PEI, and the levels of local respiratory tract and systemic immune responses were detected. In comparison to H9N2 WIV alone, antigen-specific IgA levels in local nasal cavity, trachea and lung, serum IgG and their subtype (IgG1 and IgG2a) levels, were strongly enhanced. Similarly, the activation and proliferation of splenocytes were markedly increased. In addition, PEI is superior as a H9N2 WIV delivery system due to their ability to 1): greatly increase the viral adhesion to mucosal epithelial cells; 2): enhance the cellular uptake and endosomal escape of antigens in dendritic cells (DCs), and further significantly activate DCs to mature. Taken together, these results provided more insights that PEI had potential as an adjuvant for H9N2 particle antigen intranasal vaccination.
Copyright ? 2015, American Society for Microbiology. All Rights Reserved.
PMID: 25673304 [PubMed - as supplied by publisher]
[h=1]H9N2 Influenza Whole Inactivated Virus Combined with Polyethyleneimine Strongly Enhance Mucosal and Systemic Immunity after Intranasal Immunization in Mice.[/h] Qin T[SUP]1[/SUP], Yin Y[SUP]1[/SUP], Huang L[SUP]1[/SUP], Yu Q[SUP]1[/SUP], Yang Q[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza whole inactivated virus (WIV) are more immunogenic and induce protective antibody responses than other formulations like split virus (SV) or subunit (SU) vaccines, after intranasal mucosal delivery. Polyethyleneimine (PEI), an organic polycation, is widely used as a gene transfection and DNA vaccine delivery reagent. Although PEI has potent mucosal adjuvant activity for viral subunit glycoprotein antigens recently, its immune activity with H9N2 WIV is not well demonstrated. Here, mice were immunized intranasally with H9N2 WIV combined with PEI, and the levels of local respiratory tract and systemic immune responses were detected. In comparison to H9N2 WIV alone, antigen-specific IgA levels in local nasal cavity, trachea and lung, serum IgG and their subtype (IgG1 and IgG2a) levels, were strongly enhanced. Similarly, the activation and proliferation of splenocytes were markedly increased. In addition, PEI is superior as a H9N2 WIV delivery system due to their ability to 1): greatly increase the viral adhesion to mucosal epithelial cells; 2): enhance the cellular uptake and endosomal escape of antigens in dendritic cells (DCs), and further significantly activate DCs to mature. Taken together, these results provided more insights that PEI had potential as an adjuvant for H9N2 particle antigen intranasal vaccination.
Copyright ? 2015, American Society for Microbiology. All Rights Reserved.
PMID: 25673304 [PubMed - as supplied by publisher]