tetano
Editor, Senior Moderator
Health Sci Rep
. 2023 May 18;6(5):e1275.
doi: 10.1002/hsr2.1275. eCollection 2023 May. SARS-CoV-2 antigen detection by saliva; an alternative to nasopharyngeal specimen: A cross-sectional study
Mohammad Jubair[SUP] 1 [/SUP], Sezanur Rahman[SUP] 1 [/SUP], Mst Noorjahan Begum[SUP] 1 [/SUP], Muhammad Talha[SUP] 1 [/SUP], Raisha Musarrat[SUP] 1 [/SUP], A K M Shafiqur Rahman[SUP] 2 [/SUP], Mohammed Shehab Uddin[SUP] 3 [/SUP], AiMin Wen[SUP] 4 [/SUP], YaoHui Ning[SUP] 4 [/SUP], Kamrun Nahar[SUP] 1 [/SUP], Mohammed Ziaur Rahman[SUP] 1 [/SUP], Mustafizur Rahman[SUP] 1 [/SUP]
Affiliations
Background and aims: Saliva samples are less invasive and more convenient for patients than naso- and/or oropharynx swabs (NOS). However, there is no US Food and Drug Administration-approved severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) rapid antigen test kit, which can be useful in a prolonged pandemic to reduce transmission by allowing suspected individuals to self-sampling. We evaluated the performances of High sensitive AQ[SUP]+[/SUP] Rapid SARS-CoV-2 Antigen Test (AQ[SUP]+[/SUP] kit) using nasopharyngeal swabs (NPs) and saliva specimens from the same patients in laboratory conditions.
Methods: The real-time reverse transcription-polymerase chain reaction (rRT-PCR) test result was used for screening the inrolled individuals and compared as the gold standard. NP and saliva samples were collected from 100 rRT-PCR positives and 100 negative individuals and tested with an AQ[SUP]+[/SUP] kit.
Results: The AQ[SUP]+[/SUP] kit showed good performances in both NP and saliva samples with an overall accuracy of 98.5% and 94.0%, and sensitivity of 97.0% and 88.0%, respectively. In both cases, specificity was 100%. AQ[SUP]+[/SUP] kit performance with saliva was in the range of the World Health Organization recommended value.
Conclusion: xOur findings indicate that the saliva specimen can be used as an alternative and less invasive to NPs for quick and reliable SARS-CoV-2 antigen detection.
Keywords: COVID‐19 diagnosis; SARS‐CoV‐2; kit evaluation; rapid antigen test; saliva.
. 2023 May 18;6(5):e1275.
doi: 10.1002/hsr2.1275. eCollection 2023 May. SARS-CoV-2 antigen detection by saliva; an alternative to nasopharyngeal specimen: A cross-sectional study
Mohammad Jubair[SUP] 1 [/SUP], Sezanur Rahman[SUP] 1 [/SUP], Mst Noorjahan Begum[SUP] 1 [/SUP], Muhammad Talha[SUP] 1 [/SUP], Raisha Musarrat[SUP] 1 [/SUP], A K M Shafiqur Rahman[SUP] 2 [/SUP], Mohammed Shehab Uddin[SUP] 3 [/SUP], AiMin Wen[SUP] 4 [/SUP], YaoHui Ning[SUP] 4 [/SUP], Kamrun Nahar[SUP] 1 [/SUP], Mohammed Ziaur Rahman[SUP] 1 [/SUP], Mustafizur Rahman[SUP] 1 [/SUP]
Affiliations
- PMID: 37216057
- PMCID: PMC10196438
- DOI: 10.1002/hsr2.1275
Background and aims: Saliva samples are less invasive and more convenient for patients than naso- and/or oropharynx swabs (NOS). However, there is no US Food and Drug Administration-approved severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) rapid antigen test kit, which can be useful in a prolonged pandemic to reduce transmission by allowing suspected individuals to self-sampling. We evaluated the performances of High sensitive AQ[SUP]+[/SUP] Rapid SARS-CoV-2 Antigen Test (AQ[SUP]+[/SUP] kit) using nasopharyngeal swabs (NPs) and saliva specimens from the same patients in laboratory conditions.
Methods: The real-time reverse transcription-polymerase chain reaction (rRT-PCR) test result was used for screening the inrolled individuals and compared as the gold standard. NP and saliva samples were collected from 100 rRT-PCR positives and 100 negative individuals and tested with an AQ[SUP]+[/SUP] kit.
Results: The AQ[SUP]+[/SUP] kit showed good performances in both NP and saliva samples with an overall accuracy of 98.5% and 94.0%, and sensitivity of 97.0% and 88.0%, respectively. In both cases, specificity was 100%. AQ[SUP]+[/SUP] kit performance with saliva was in the range of the World Health Organization recommended value.
Conclusion: xOur findings indicate that the saliva specimen can be used as an alternative and less invasive to NPs for quick and reliable SARS-CoV-2 antigen detection.
Keywords: COVID‐19 diagnosis; SARS‐CoV‐2; kit evaluation; rapid antigen test; saliva.