tetano
Editor, Senior Moderator
AIDS Res Hum Retroviruses. 2015 Aug 31. [Epub ahead of print]
[h=1]Heterogeneity of T Cell Responses to Pandemic pH1N1 Monovalent Vaccine in HIV-Infected Pregnant Women.[/h] Weinberg A[SUP]1[/SUP], Muresan P[SUP]2[/SUP], Richardson K[SUP]1[/SUP], Fenton T[SUP]2[/SUP], Dominguez T[SUP]1[/SUP], Bloom A[SUP]3[/SUP], Watts DH[SUP]4[/SUP], Abzug MJ[SUP]1[/SUP], Nachman SA[SUP]5[/SUP], Levin MJ[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] We investigated the Th1 protective and regulatory T and B cell (Treg and Breg) responses to pH1N1 monovalent influenza vaccine (IIV1) in HIV-infected pregnant women on combination antiretroviral therapy (cART). Peripheral blood mononuclear cells (PBMCs) from 52 study participants were cryopreserved before and after vaccination and analyzed by flow cytometry. pH1N1-specific Th1, Treg, and Breg responses were measured in PBMCs after in vitro stimulation with pH1N1 and control antigen. The cohort analysis did not detect changes in pH1N1-Th1, Treg, or Breg subsets postvaccination. However, individual analyses distinguished subjects who mounted vigorous Th1 responses postvaccination from others who did not. Postvaccination, high pH1N1-Th1 correlated with high pH1N1-Treg and Breg responses, suggesting that low influenza effector responses did not result from excessive vaccine-induced immune regulation. High postvaccination pH1N1-Th1 responses correlated with baseline high PHA- and pH1N1-IFN-γ ELISpot and circulating CD4[SUP]+[/SUP]CD39[SUP]+[/SUP]% and CD8[SUP]+[/SUP]CD39[SUP]+[/SUP]% Treg, with low CD8[SUP]+[/SUP] cell numbers and CD19[SUP]+[/SUP]FOXP3[SUP]+[/SUP]% Breg, but not with CD4[SUP]+[/SUP] cell numbers or HIV viral load. These data highlight the heterogeneity of T cell responses to vaccines in HIV-infected individuals on cART. Predictors of robust Th1 responses to IIV include CD8[SUP]+[/SUP] cell numbers, T cell functionality, and circulating Breg and Treg.
PMID: 26322930 [PubMed - as supplied by publisher]
[h=1]Heterogeneity of T Cell Responses to Pandemic pH1N1 Monovalent Vaccine in HIV-Infected Pregnant Women.[/h] Weinberg A[SUP]1[/SUP], Muresan P[SUP]2[/SUP], Richardson K[SUP]1[/SUP], Fenton T[SUP]2[/SUP], Dominguez T[SUP]1[/SUP], Bloom A[SUP]3[/SUP], Watts DH[SUP]4[/SUP], Abzug MJ[SUP]1[/SUP], Nachman SA[SUP]5[/SUP], Levin MJ[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] We investigated the Th1 protective and regulatory T and B cell (Treg and Breg) responses to pH1N1 monovalent influenza vaccine (IIV1) in HIV-infected pregnant women on combination antiretroviral therapy (cART). Peripheral blood mononuclear cells (PBMCs) from 52 study participants were cryopreserved before and after vaccination and analyzed by flow cytometry. pH1N1-specific Th1, Treg, and Breg responses were measured in PBMCs after in vitro stimulation with pH1N1 and control antigen. The cohort analysis did not detect changes in pH1N1-Th1, Treg, or Breg subsets postvaccination. However, individual analyses distinguished subjects who mounted vigorous Th1 responses postvaccination from others who did not. Postvaccination, high pH1N1-Th1 correlated with high pH1N1-Treg and Breg responses, suggesting that low influenza effector responses did not result from excessive vaccine-induced immune regulation. High postvaccination pH1N1-Th1 responses correlated with baseline high PHA- and pH1N1-IFN-γ ELISpot and circulating CD4[SUP]+[/SUP]CD39[SUP]+[/SUP]% and CD8[SUP]+[/SUP]CD39[SUP]+[/SUP]% Treg, with low CD8[SUP]+[/SUP] cell numbers and CD19[SUP]+[/SUP]FOXP3[SUP]+[/SUP]% Breg, but not with CD4[SUP]+[/SUP] cell numbers or HIV viral load. These data highlight the heterogeneity of T cell responses to vaccines in HIV-infected individuals on cART. Predictors of robust Th1 responses to IIV include CD8[SUP]+[/SUP] cell numbers, T cell functionality, and circulating Breg and Treg.
PMID: 26322930 [PubMed - as supplied by publisher]